MicroRNA-JAK/STAT回路による昼間行動出力の調節
1Cell and Molecular Biology Program, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA.
Cell
|February 7, 2012
まとめ
マイクロRNAmiR-279は,JAK/STAT経路を調節することにより,フルーツハエの毎日の休息:活動リズムを制御し,中央日経時計の下流に作用して行動に影響を与えます.
科学分野:
- クロノバイオロジーはクロノバイオロジーを用います.
- 分子生物学は分子生物学である.
- 神経科学は神経科学である.
背景:
- 昼間の時計の分子機構は知られているが,それがどのようにリズム的な行動を生成するのかは不明である.
- 昼間の時計からの信号伝達を理解することは,行動リズムを説明するために不可欠です.
研究 の 目的:
- ドロソフィラの昼夜リズムと行動の調節におけるマイクロRNA miR-279の役割を調査する.
- miR-279が休息:活動パターンに影響を与える分子経路を解明する.
主な方法:
- ドロソフィラのmiR-279発現 (過剰発現と消去) の遺伝子操作.
- 休息の分析:活動リズムと昼夜行動.
- リガンド Upd.を含むJAK/STAT信号経路の調査.
- 時計ニューロンとUpd発現ニューロンの関係に関する研究.
主要な成果:
- miR-279は,Drosophilaの休息:活動リズムを駆動するために,JAK/STAT信号を調節する.
- miR-279のレベルを変化させると (過剰発現または消去) これらのリズムが乱されます.
- miR-279は,PERIODタンパク質の振動が正常のままであるため,中央の昼夜時計の下流で作用します.
- JAK/STATのリガンドUpdはmiR-279の直接標的であり,そのノックダウンによりmiR-279の変異性フェノタイプを救出する.
- JAK/STAT経路の調節は昼夜リズムに影響を与え,中央時計ニューロンはUpd発現ニューロンと物理的に相互作用する可能性があります.
結論:
- マイクロRNA miR-279は,ドロソフィラの昼休み:活動リズムを調節する重要なレギュラーである.
- miR-279/JAK/STAT経路は,行動制御のための中央日経時計のためのメカニズムを提供します.
- この研究は,マイクロRNA,JAK/STAT経路,および昼夜リズム生成の間の新しいリンクを明らかにしています.
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