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Long-patch Base Excision Repair01:02

Long-patch Base Excision Repair

Since the discovery of the two BER pathways, there has been a debate about how a cell chooses one pathway over the other and the factors determining this selection. Numerous in vitro experiments have pointed out multiple determinants for the sub-pathway selection. These are:
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
Gene Conversion02:08

Gene Conversion

Other than maintaining genome stability via DNA repair, homologous recombination plays an important role in diversifying the genome. In fact, the recombination of sequences forms the molecular basis of genomic evolution. Random and non-random permutations of genomic sequences create a library of new amalgamated sequences. These newly formed genomes can determine the fitness and survival of cells. In bacteria, homologous and non-homologous types of recombination lead to the evolution of new...
Gene Conversion02:08

Gene Conversion

Other than maintaining genome stability via DNA repair, homologous recombination plays an important role in diversifying the genome. In fact, the recombination of sequences forms the molecular basis of genomic evolution. Random and non-random permutations of genomic sequences create a library of new amalgamated sequences. These newly formed genomes can determine the fitness and survival of cells. In bacteria, homologous and non-homologous types of recombination lead to the evolution of new...
Fixing Double-strand Breaks02:04

Fixing Double-strand Breaks

The double-stranded structure of DNA has two major advantages. First, it serves as a safe repository of genetic information where one strand serves as the back-up in case the other strand is damaged. Second, the double-helical structure can be wrapped around proteins called histones to form nucleosomes, which can then be tightly wound to form chromosomes. This way, DNA chains up to 2 inches long can be contained within microscopic structures in a cell. A double-stranded break not only damages...
Homologous Recombination02:31

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...

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Updated: May 25, 2026

Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae
07:55

Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae

Published on: September 11, 2022

完全互補の3方向のDNA結合のブランチポイントの拡張.

Tara Sabir1, Anita Toulmin, Long Ma

  • 1The School of Chemistry, Alan Turing Building, The University of Manchester, Oxford Road, Manchester M13 9PL, UK.

Journal of the American Chemical Society
|February 15, 2012
PubMed
まとめ

研究者はDNAの三方向結合 (3WJs) を研究し,それらは内部空洞を持つY形構造を形成することを発見しました. この発見は,ナノサイエンスの応用のためのDNA構造の理解を前進させる.

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Generating Transgenic Plants with Single-copy Insertions Using BIBAC-GW Binary Vector
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Generating Transgenic Plants with Single-copy Insertions Using BIBAC-GW Binary Vector

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Rapid Assembly of Multi-Gene Constructs using Modular Golden Gate Cloning
08:31

Rapid Assembly of Multi-Gene Constructs using Modular Golden Gate Cloning

Published on: February 5, 2021

関連する実験動画

Last Updated: May 25, 2026

Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae
07:55

Detection of Homologous Recombination Intermediates via Proximity Ligation and Quantitative PCR in Saccharomyces cerevisiae

Published on: September 11, 2022

Generating Transgenic Plants with Single-copy Insertions Using BIBAC-GW Binary Vector
12:08

Generating Transgenic Plants with Single-copy Insertions Using BIBAC-GW Binary Vector

Published on: March 28, 2018

Rapid Assembly of Multi-Gene Constructs using Modular Golden Gate Cloning
08:31

Rapid Assembly of Multi-Gene Constructs using Modular Golden Gate Cloning

Published on: February 5, 2021

科学分野:

  • 分子生物学は分子生物学である.
  • バイオフィジックス 生物物理学
  • ナノサイエンス ナノ科学

背景:

  • 枝分かれした核酸は,DNAの三方向結合 (3WJs) のように,DNA複製,再結合,修復などの生物学的プロセスにおいて極めて重要です.
  • これらの構造は,RNAアーキテクチャやナノサイエンスにおける構成要素としても応用されます.

研究 の 目的:

  • 溶液中のDNA三方向結合 (3WJs) の局所的および全局的構造を解明する.
  • 3WJsの観察された柔軟性と反応性の構造的根拠を理解する.

主な方法:

  • 高解像度単分子フォースター共振エネルギー転送 (FRET)
  • 時間解像度スペクトロスコピーは,時間解像度スペクトロスコピーを用います.
  • 分子モデリング

主要な成果:

  • DNA 3WJは溶液中のピラミッド状のY形状の形状を採用しています.
  • ブランチポイントに隣接するベースは,ワトソン・クリックの完全な互補性にもかかわらず,ペア化されていないままです.
  • この分離は,交差点の中心にナノスケールの空洞を作り出します.

結論:

  • 判定された構造は,3WJの柔軟性と反応性に関する以前の観察を説明する.
  • これらの発見は,新しいDNAベースの超分子受容体およびナノシステムの設計のための構造的基礎を提供します.