関連する実験動画
Updated: May 8, 2026

11:36
In Vitro Ubiquitination and Deubiquitination Assays of Nucleosomal Histones
Published on: July 25, 2019
COPIIコートのサイズと機能のウビキチン依存の調節
Lingyan Jin1, Kanika Bajaj Pahuja, Katherine E Wickliffe
1Department of Molecular and Cell Biology, University of California at Berkeley, California 94720, USA.
Nature
|February 24, 2012
まとめ
Ubiquitin ligase CUL3-KLHL12 monoubiquitylates SEC31は,コラーゲンのような大容量の貨物に対して,より大きなCOPIIベシクルコートを可能にします. この発見は,効率的なタンパク質分泌のためにCOPII膀の大きさがどのように調節されているかを説明します.
科学分野:
- 細胞生物学 細胞生物学
- 分子生物学と細胞生物学について
- バイオケミストリー バイオケミストリー
背景:
- エンドプラズマ網膜からCOPII膀経由のタンパク質輸送は,細胞分泌に不可欠である.
- ほとんどのCOPIIベシクルは60〜80nmですが,大容量の貨物 (例えば,プロコラーゲン繊維,キロミクロン) についてはより大きなものが必要です.
- COPII小胞形成の欠陥は,コラーゲン堆積障害やキロミクロン保持疾患などの疾患を引き起こす.
研究 の 目的:
- COPII小胞の大きさを制御するメカニズムを解明する.
- COPIIコート組立と貨物特有の輸送の規制者を特定する.
主な方法:
- COPII小胞形成におけるユビキチンリガゼCUL3-KLHL12の役割を調査した.
- COPIIコンポーネント SEC31.1.の普遍性を分析しました.
- CUL3-KLHL12がCOPIIのコートサイズと貨物輸送に与える影響を評価した.
主要な成果:
- CUL3-KLHL12をCOPIIコートアセンブリの重要なレギュレータとして特定しました.
- CUL3-KLHL12がSEC31モノウビキチレーションを触媒化し,より大きなCOPIIコートの形成を促進することを実証しました.
- CUL3-KLHL12媒介の汎用化は,コラーゲン輸出には不可欠であるが,小型の貨物輸送にはそれほど重要ではないことが示された.
結論:
- CUL3-KLHL12によるモノウビキチル化は,COPII 膀のコートサイズを制御する.
- このメカニズムは,小さなタンパク質から大きな繊維まで,多様な貨物の規制された輸送を可能にします.
- 発見は,膀の密輸と,タンパク質分泌の欠陥に関連した疾患の病原性についての洞察を提供します.
関連する概念動画
Covalently Linked Protein Regulators
Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein.
These groups modify specific amino acids in a protein.
Regulated Protein Degradation
It is vital to regulate the activity of enzymatic as well as non-enzymatic proteins inside the cell. This can be achieved either through creating a balance between their rate of synthesis and degradation or regulating the intrinsic activity of the protein. Both these regulation mechanisms play an essential role in the normal functioning of cells.
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...
Coat Assembly and GTPases
Vesicles incorporate different coat protein subunits in different cell locations, which changes the properties of the coat, such as the shape and geometry of the transport vesicles. Thus, vesicle coat proteins also play a significant role in cargo selection.
Coat assembly depends on the local availability of phosphatidylinositol phosphates or PIPs and GTP-binding proteins. Adaptor proteins, which link the coat proteins to the membrane, bind to these PIPs and play a crucial role in controlling...
Coat assembly depends on the local availability of phosphatidylinositol phosphates or PIPs and GTP-binding proteins. Adaptor proteins, which link the coat proteins to the membrane, bind to these PIPs and play a crucial role in controlling...
Regulation of the Unfolded Protein Response
Inositol-requiring kinase one or IRE1 is the most conserved eukaryotic unfolded protein response (UPR) receptor. It is a type I transmembrane protein kinase receptor with a distinctive site-specific RNase activity. As the binding mechanics of the misfolded proteins with the N-terminal domain of IRE-1 are unclear, three binding models — direct, indirect, and allosteric -- are proposed for receptor activation. Nevertheless, it is known that once a misfolded protein associates with IRE1, it...
Covalently Linked Protein Regulators
Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein.
These groups modify specific amino acids in a protein.
Regulated Protein Degradation
It is vital to regulate the activity of enzymatic as well as non-enzymatic proteins inside the cell. This can be achieved either through creating a balance between their rate of synthesis and degradation or regulating the intrinsic activity of the protein. Both these regulation mechanisms play an essential role in the normal functioning of cells.
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...

