ニューロペプチドがシナプスに届くのは,長距離の膀循環と散発的な捕獲によるものです

Man Yan Wong¹, Chaoming Zhou, Dinara Shakiryanova

¹Department of Pharmacology and Chemical Biology, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA.

Cell

|March 6, 2012

まとめ

濃厚なコアベシクル (DCV) は,遠隔の標的に到達するためにいくつかのサイトを回避し,双方向輸送を通じて軸索に循環します. この膀循環は,すべての神経放出部位に均一な供給を保証する.

科学分野:

神経科学は神経科学である.
細胞生物学細胞生物学

背景:

軸索輸送は神経伝達に不可欠であり,神経端末に密集コアベシクル (DCV) のような重要な成分を供給します.
DCVを順次放出場所 (en passant boutons) に一方向的な前向き輸送によって均一に分配することは,物流上の課題です.

研究の目的:

ドロソフィラの軸索内のDCVの輸送ダイナミクスを調査する.
DCVがどのように均一にen passantボタンに供給されているかを理解するために.

主な方法:

ドロソフィラの同時光白化と画像撮影 (SPAIM) を用いた神経ペプチドを含むDCVのインビボ追跡.
アントログラードおよびレトログラード輸送を含むDCV移動パターンの分析.

主要な成果:

アントログラド型DCVは,優先的に最も遠端のブートンに蓄積し,近隣のブートンをバイパスします.
過剰なディスタルDCVは,ダイナクチン依存の逆行輸送を経て,ソマへ戻ります.
DCVは双方向の循環を示し,反復的な前向き輸送のために方向を逆転させ,散発的に通り過ぎるボタンで捕獲されます.

結論:

遠距離逆行輸送と双方向捕獲を含む膀循環は,単純な前行輸送の限界を克服する.
このダイナミックな循環メカニズムは,軸索に沿ったすべての通過放出部位に効率的で均等なDCV供給を確保します.

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