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Updated: May 24, 2026

Anaerobic Protein Purification and Kinetic Analysis via Oxygen Electrode for Studying DesB Dioxygenase Activity and Inhibition
08:31

Anaerobic Protein Purification and Kinetic Analysis via Oxygen Electrode for Studying DesB Dioxygenase Activity and Inhibition

Published on: October 3, 2018

毒性のあるエポキシドを形成し,脱酸素化する酸素酵素である

Robin Teufel1, Thorsten Friedrich, Georg Fuchs

  • 1Mikrobiologie, Fakultät Biologie, Universität Freiburg, Schänzlestrasse 1, D-79104 Freiburg, Germany.

Nature
|March 9, 2012
PubMed
まとめ

この研究では,細菌の酵素複合体であるPaaABCEが,意外に有毒なエポキシドを脱酸素化し,細胞損傷を防ぐことが明らかになりました. この二機能性オキシゲネーゼ/デオキシゲネーゼは,代謝解毒経路に関する新しい洞察を提供します.

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Benchtop Immobilized Metal Affinity Chromatography, Reconstitution and Assay of a Polyhistidine Tagged Metalloenzyme for the Undergraduate Laboratory08:02

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