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Updated: May 24, 2026

Combining Non-reducing SDS-PAGE Analysis and Chemical Crosslinking to Detect Multimeric Complexes Stabilized by Disulfide Linkages in Mammalian Cells in Culture
09:37

Combining Non-reducing SDS-PAGE Analysis and Chemical Crosslinking to Detect Multimeric Complexes Stabilized by Disulfide Linkages in Mammalian Cells in Culture

Published on: May 2, 2019

構造とダイナミクスの調節は,展開状態でのディスルファイド結合形成によって行われます

Robert Silvers1, Friederike Sziegat, Hideki Tachibana

  • 1Institute for Organic Chemistry and Chemical Biology, Center of Biomolecular Magnetic Resonance, Goethe University Frankfurt, Max-von-Laue-Strasse 7, 60438 Frankfurt am Main, Germany.

Journal of the American Chemical Society
|March 15, 2012
PubMed
まとめ

単一二酸化硫化物結合は,タンパク質の折り畳み動態を大幅に変化させます. ディスルファイド結合の1つを導入すると,ネイティブ状態に匹敵する秩序ある構造が作られ,水害性コア残留物の行動に影響を与えます.

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