循環型AMP受容体は,ホルモン依存性乳腺腫瘍細胞フリーシステムにおける核タンパク質のリン酸化を誘発する
Science (New York, N.Y.)
|September 28, 1979
まとめ
特定の循環型AMP受容体タンパク質が乳腺腫瘍で増加する. このタンパク質は核に移動し,核タンパク質のリン酸化を支援し,ホルモン依存性乳腺腫瘍におけるその役割を示唆しています.
科学分野:
- 分子生物学は分子生物学である.
- がん研究 がん研究
- バイオケミストリー バイオケミストリー
背景:
- ホルモン依存性乳腺腫瘍は,複雑な分子変化を示す.
- アデノシン3',5'-モノフォスファート (循環型AMP) 信号伝達経路は,細胞の調節において極めて重要です.
研究 の 目的:
- 7,12-ジメチルベンズ[a]アントラゼン (DMBA) 誘発性乳腺腫瘍における循環型AMP受容体タンパク質の役割を調査する.
- これらの腫瘍における循環型AMP受容体を含む核事象を解明する.
主な方法:
- 循環型AMP,ベンザミド,アルギニンによるDMBA誘発乳腺腫瘍切片のインキュベーション.
- 腫瘍細胞塩基を腫瘍核とインキュベーションし,核吸収とタンパク質リン酸化を評価する.
- 牛の心臓タンパク質キナーゼII型を用いて核結合とリン酸化を研究する.
主要な成果:
- 56,000ダルトンの循環型AMP受容体タンパク質は,DMBA誘発の乳腺腫瘍の潜伏後において有意に増加した.
- 56,000ダルトンの受容体は核吸収を受け,76,000ダルトンの核タンパク質のリン酸化を促進しました.
- また,外因性タンパク質キナーゼII型を用いたDMBA腫瘍核における受容体結合とタンパク質リン酸化も観察された.
結論:
- 56,000ダルトンサイクリックAMP受容体タンパク質は,ホルモン依存性乳腺腫瘍の核発生に作用する.
- 循環型AMP受容体の核プロセスへの関与は,乳腺腫瘍発達の潜在的なメカニズムを示唆する.
関連する概念動画
01:12What are Second Messengers?
Because many receptor binding ligands are hydrophilic, they do not cross the cell membrane and thus their message must be relayed to a second messenger on the inside. There are several second messenger pathways, each with their own way of relaying information. G-protein coupled receptors can activate both phosphoinositol and cyclic AMP (cAMP) second messenger pathways. The phosphoinositol path is active when the receptor induces phospholipase C to hydrolyze the phospholipid,...
01:24Intracellular Signaling Cascades
Once a ligand binds to a receptor, the signal is transmitted through the membrane and into the cytoplasm. The continuation of a signal in this manner is called signal transduction. Signal transduction only occurs with cell-surface receptors, which cannot interact with most components of the cell, such as DNA. Only internal receptors can interact directly with DNA in the nucleus to initiate protein synthesis. When a ligand binds to its receptor, conformational changes occur that affect the...
02:38Mitogens and the Cell Cycle
Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...
01:15Amplifying Signals via Second Messengers
Many receptor binding ligands are hydrophilic; they do not cross the cell membrane but bind to cell-surface receptors. Thus, their message must be relayed by second messengers present in the cell cytoplasm. There are several second messenger pathways, each with its own way of relaying information. For example, the G protein-coupled receptors can activate both phosphoinositol and cyclic AMP (cAMP) second messenger pathways. The phosphoinositol pathway is active when the receptor induces...
01:22Amplifying Signals via Enzymatic Cascade
When a ligand binds to a cell-surface receptor, the receptor's intracellular domain changes shape, which may either activate its enzyme function or allow its binding to other molecules. The initial signal is amplified by most signal transduction pathways. This means that a single ligand molecule can activate multiple molecules of a downstream target. Proteins that relay a signal are most commonly phosphorylated at one or more sites, activating or inactivating the protein. Kinases catalyze the...
01:25cAMP-dependent Protein Kinase Pathways
Cyclic Adenosine Monophosphate (cAMP) is an essential second messenger that activates protein kinase A (PKA) and regulates various biological processes. A single epinephrine molecule binds to GPCR and activates several heterotrimeric G proteins, each stimulating multiple adenylyl cyclase, amplifying the signal, and synthesizing large numbers of cAMP molecules. Small changes in cAMP concentration affect PKA activity. The binding of four cAMP molecules induces a conformational change in PKA,...