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遺伝子発現ノイズを使用して,遺伝子調節を理解する
Brian Munsky1, Gregor Neuert, Alexander van Oudenaarden
1Center for Nonlinear Studies, the National Flow Cytometry Resource, Los Alamos National Laboratory, Los Alamos, NM 87545, USA. munsky@lanl.gov
Science (New York, N.Y.)
|April 14, 2012
まとめ
遺伝子発現の騒音から生じる生物学的多様性は,同一の細胞でも生じる. この多様性を測定することで,遺伝子調節メカニズムとダイナミクスの洞察が得られます.
科学分野:
- 分子生物学は分子生物学である.
- システム生物学 システム生物学
- 遺伝学 遺伝学とは
背景:
- フェノタイプ的変異は,遺伝的および環境的要因に起因する生物学の共通点です.
- 遺伝的に同一の細胞でさえ,同一の環境の中で,現象型的な違いを示す.
- ストカスティック遺伝子発現,または遺伝子発現ノイズは,この変動性の重要な要因です.
研究 の 目的:
- 遺伝子発現の変動性が遺伝子調節を理解するためにどのように使用されているかを検討する.
- 騒音を用いた遺伝子調節メカニズムを定量的に探求する研究を強調する.
- 遺伝子調節の根本的な問題における騒音の役割を強調する.
主な方法:
- タンパク質とメッセンジャーRNAのレベルにおける細胞間変動の分析.
- 実験的な測定をディスクリートストキャスティックモデルと統合する.
- 遺伝子発現の変動性を研究ツールとして採用した最近の研究のレビュー.
主要な成果:
- 遺伝子発現ノイズと遺伝子調節メカニズムとの間に強い相関が発見されました.
- 変性測定は,遺伝子調節を調査するための敏感な方法を提供します.
- 遺伝子調節のダイナミクスの定量的な理解は,ノイズ分析によって進歩しています.
結論:
- 遺伝子発現の騒音は,生物学的多様性の重要な源である.
- 騒音の測定とモデリングは,遺伝子調節の研究に強力なアプローチを提供します.
- 変化度測定とストキャスティックモデルを統合したさらなる研究は,遺伝子規制ネットワークの理解を深めるでしょう.
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