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関連する概念動画

RNA Structure01:19

RNA Structure

The basic structure of RNA consists of a string of ribonucleotides attached by phosphodiester bonds. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...
RNA Structure01:23

RNA Structure

Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
RNA Structure01:23

RNA Structure

Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
Nucleic Acid Structure01:25

Nucleic Acid Structure

The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms  a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA has a double-helix structure. The...
Protein Folding01:22

Protein Folding

Overview
Protein Folding01:22

Protein Folding

Overview

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関連する実験動画

Updated: May 23, 2026

Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen
11:32

Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen

Published on: May 24, 2017

協同的三次相互作用ネットワークは,RNAの折り畳みを導く.

Reza Behrouzi1, Joon Ho Roh, Duncan Kilburn

  • 1T.C. Jenkins Department of Biophysics, Johns Hopkins University, 3400 North Charles Street, Baltimore, MD 21218, USA.

Cell
|April 17, 2012
PubMed
まとめ

非コーディングRNAは,個々の三次接触ではなく,協力的な相互作用を通じてユニークな3D構造を達成します. この協同性はRNAの折り畳みを誘導し,安定したネイティブ状態のために不正な構造を抑制します.

科学分野:

  • バイオケミストリー バイオケミストリー
  • 分子生物学は分子生物学である.
  • 構造生物学 構造生物学とは

背景:

  • 非コーディングRNAは,規制機能に不可欠な複雑な3D構造に折りたたまれます.
  • RNAの折り畳みメカニズム,特に三次相互作用の役割を理解することは極めて重要です.

研究 の 目的:

  • 非コーディングRNAが,限られた三次相互作用モチーフを持つユニークな3D構造をどのように達成するかを調査する.
  • RNAの折り畳みとネイティブ状態の形成における協力性の役割を明らかにする.

主な方法:

  • グループIのリボジームにおける三次相互作用のサイト誘導性変異.
  • 小角X線散射 (SAXS) により,構造的混乱を検出する.
  • 酵素活性アッセイ,ヒドロキシルラジカルフットプリント,ネイティブポリアクリラミドゲル電泳 (PAGE) で折り畳みと安定性を評価する.

主要な成果:

  • ほとんどの三次相互作用は,原生状態の安定性に最小限の影響を及ぼした.
  • 折り畳み可能な中間材料では,コアと周辺の構造モチーフの間の協力的リンクが観察されました.
  • この協同性は,ネイティブヘリックス・オリエンテーションに依存し,非ネイティブ構造を抑制した.

さらに関連する動画

Nanomanipulation of Single RNA Molecules by Optical Tweezers
06:59

Nanomanipulation of Single RNA Molecules by Optical Tweezers

Published on: August 20, 2014

Optical Tweezers to Study RNA-Protein Interactions in Translation Regulation
12:26

Optical Tweezers to Study RNA-Protein Interactions in Translation Regulation

Published on: February 12, 2022

関連する実験動画

Last Updated: May 23, 2026

Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen
11:32

Mapping RNA-RNA Interactions Globally Using Biotinylated Psoralen

Published on: May 24, 2017

Nanomanipulation of Single RNA Molecules by Optical Tweezers
06:59

Nanomanipulation of Single RNA Molecules by Optical Tweezers

Published on: August 20, 2014

Optical Tweezers to Study RNA-Protein Interactions in Translation Regulation
12:26

Optical Tweezers to Study RNA-Protein Interactions in Translation Regulation

Published on: February 12, 2022

結論:

  • RNAの折り畳みは,個々の三次相互作用によってのみ導かれるのではなく,新たな協力的な相互作用ネットワークによって導かれる.
  • 協同性は,非コーディングRNAにおけるユニークで安定したネイティブフォールドの形成を保証するために進化した可能性が高い.