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関連する概念動画

Microtubule Formation01:23

Microtubule Formation

Microtubules are dynamic structures that undergo continuous assembly and disassembly. They originate from specialized multi-protein complexes known as microtubule organizing centers or MTOCs. Within the MTOC, the point of origin of the microtubule is known as the minus end, while the end radiating outward is the plus end. Microtubules serve two primary functions — the organization of spindle complexes to separate sister chromatids during mitotic or meiotic cell division and the formation of...
Assembly of Complex Microtubule Structures01:32

Assembly of Complex Microtubule Structures

Complex microtubule structures are present in resting cells and in dividing cells. In resting cells, they are responsible for maintaining the cellular architecture, tracks for intracellular transport, positioning of organelles, assembly of cilia and flagella. They mediate the bipolar spindle assembly for chromosomal segregation and positioning of the cell division plate in dividing cells. The formation of microtubule complex structures depends on the cell type, cell stage, and cell function.
Microtubule Instability02:17

Microtubule Instability

Microtubules are hollow cylindrical filaments having a diameter of approximately 25 nm and a length that varies from 200 nm to 25 μm. GTP-bound tubulin subunits form αβ-heterodimers for microtubule assembly. These core building blocks interact longitudinally, polymerizing into protofilaments. The protofilaments then interact with one another through lateral bonding forces to form stable cylindrical microtubules. These cylindrical filaments are dynamic as they undergo repeated assembly and...
Microtubule Instability02:17

Microtubule Instability

Microtubules are hollow cylindrical filaments having a diameter of approximately 25 nm and a length that varies from 200 nm to 25 μm. GTP-bound tubulin subunits form αβ-heterodimers for microtubule assembly. These core building blocks interact longitudinally, polymerizing into protofilaments. The protofilaments then interact with one another through lateral bonding forces to form stable cylindrical microtubules. These cylindrical filaments are dynamic as they undergo repeated assembly and...
Coat Assembly and GTPases01:33

Coat Assembly and GTPases

Vesicles incorporate different coat protein subunits in different cell locations, which changes the properties of the coat, such as the shape and geometry of the transport vesicles. Thus, vesicle coat proteins also play a significant role in cargo selection.
Coat assembly depends on the local availability of phosphatidylinositol phosphates or PIPs and GTP-binding proteins. Adaptor proteins, which link the coat proteins to the membrane, bind to these PIPs and play a crucial role in controlling...
Microtubule Associated Proteins (MAPs)01:42

Microtubule Associated Proteins (MAPs)

Microtubule function and architecture are regulated by an array of specialized proteins called microtubule-associated proteins or MAPs. These proteins are widespread across different organisms and have conserved protein motifs, like the multi-TOG domain for tubulin binding found in the CLASP family of MAPs. Some MAPs are lineage-specific based on their conserved domains. Their functions depend upon the cytoskeletal architecture and cell type they are located within. In-plant cells, a specific...

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関連する実験動画

Updated: May 23, 2026

Extracting Modified Microtubules from Mammalian Cells to Study Microtubule-Protein Complexes by Cryo-Electron Microscopy
08:02

Extracting Modified Microtubules from Mammalian Cells to Study Microtubule-Protein Complexes by Cryo-Electron Microscopy

Published on: March 3, 2023

EBは,成長するマイクロチューブルの端にある核酸依存の構造的キャップを認識します.

Sebastian P Maurer1, Franck J Fourniol, Gergő Bohner

  • 1Cancer Research UK London Research Institute, Lincoln's Inn Fields Laboratories, 44 Lincoln's Inn Fields, London WC2A 3LY, UK.

Cell
|April 17, 2012
PubMed
まとめ

エンド・バインディングタンパク質 (EBs) は,特定の構造に結合することによって,成長するマイクロチューブルの末端を追跡します. この研究は,Mal3 EBがどのように進化したかを明らかにしています.

科学分野:

  • 細胞生物学 細胞生物学
  • 構造生物学 構造生物学とは
  • バイオケミストリー バイオケミストリー

背景:

  • 成長するマイクロチューブル末端は,マイクロチューブルダイナミクスを調節するタンパク質の重要な結合部位である.
  • 末端結合タンパク質 (EBs) は,これらのダイナミックな微小管末端を識別し,結合し,他の要因を勧誘する.
  • 成長するマイクロチューブル末端のEB認識の正確な構造的基礎は,ほとんど不明のままです.

研究 の 目的:

  • 分裂酵母EB Mal3のカルポニンホモロジー (CH) ドメインが成長するマイクロチューブルの端にどのように結合するかの擬原子モデルを決定する.
  • EBsによって認識される微小管末端領域の構造的特徴を明らかにする.
  • マイクロチューブルの末端構造,EB結合,およびマイクロチューブルのダイナミクスとの関係を理解する.

主な方法:

  • 高解像度構造決定のための冷凍電子顕微鏡 (冷凍-EM).
  • サブナノメートルの単粒子の再構築で,擬原子モデルを構築する.
  • 束縛とダイナミクスをin situで検証するための光成像.

主要な成果:

  • 成長するマイクロチューブル末端に結合したMal3 CHドメインの擬原子モデルが生成されました.

さらに関連する動画

Preparation of Segmented Microtubules to Study Motions Driven by the Disassembling Microtubule Ends
12:20

Preparation of Segmented Microtubules to Study Motions Driven by the Disassembling Microtubule Ends

Published on: March 15, 2014

Using plusTipTracker Software to Measure Microtubule Dynamics in Xenopus laevis Growth Cones
07:32

Using plusTipTracker Software to Measure Microtubule Dynamics in Xenopus laevis Growth Cones

Published on: September 7, 2014

関連する実験動画

Last Updated: May 23, 2026

Extracting Modified Microtubules from Mammalian Cells to Study Microtubule-Protein Complexes by Cryo-Electron Microscopy
08:02

Extracting Modified Microtubules from Mammalian Cells to Study Microtubule-Protein Complexes by Cryo-Electron Microscopy

Published on: March 3, 2023

Preparation of Segmented Microtubules to Study Motions Driven by the Disassembling Microtubule Ends
12:20

Preparation of Segmented Microtubules to Study Motions Driven by the Disassembling Microtubule Ends

Published on: March 15, 2014

Using plusTipTracker Software to Measure Microtubule Dynamics in Xenopus laevis Growth Cones
07:32

Using plusTipTracker Software to Measure Microtubule Dynamics in Xenopus laevis Growth Cones

Published on: September 7, 2014

  • Mal3 CHドメインは,シームを除くマイクロチューブルのプロトフィラメントをブリッジしていることが観察されました.
  • 結合はGTP結合部位の近くで発生し,ニュクレオチド状態に対する感受性を示唆する.

    • 結論:

    • この構造は,EBがダイナミックな微小管末端を認識し,結合する方法を示しています.
    • EB結合は微小管の核酸結合部位と空間的に結びつき,その状態を潜在的に感知する.
    • これは,微小管の動的不安定性とEBsのエンドトラッキングメカニズムとの間の構造的リンクを提供します.