塩漬けのエビアルテミア (Artemia) のポリマーグロビンの進化
A M Manning1, C N Trotman, W P Tate
1Department of Biochemistry, University of Otago, Dunedin, New Zealand.
Nature
|December 13, 1990
まとめ
塩漬けのエビのような無脊椎動物は,溶融グロービン遺伝子によって形成されたポリメリックヘモグロビンを持っています. 分析により,これらの遺伝子は複数の機能ドメインをコードし,ヘモグロビン進化における古代の遺伝子融合イベントを示唆していることが明らかになった.
科学分野:
- バイオケミストリー バイオケミストリー
- 進化生物学の進化生物学について
- 分子生物学は分子生物学である.
背景:
- 脊椎動物は,ポリペプチドごとに複数のヘム結合ドメインを持つポリメリック形態を含む多様なヘモグロビン構造を示します.
- これらのポリメリックヘモグロビンは,祖先のモノメリックグロービン遺伝子の融合から生じる可能性があります.
研究 の 目的:
- 塩水エビのアルテミアから得られたポリメリックグロービン遺伝子のトランスクリプトを分析する.
- アルテミアのポリメリックヘモグロビンの構造と進化的起源を解明する.
主な方法:
- 補完的なDNA (cDNA) のクローンによる,アルテミア二次ヘモグロビンのサブユニットをコードする分析.
- タンパク質構造と進化史を予測するために,派生アミノ酸配列のバイオ情報分析.
主要な成果:
- アルテミアのグロービンメッセンジャーRNAは,特定のペプチド配列で結びついている9つのミオグロビンのようなドメインをコードします.
- 各ドメインには保存されたヘム結合残留物が含まれており,ミオグロビンのような折りたたみを採用すると予測されています.
- 配列分析により,これらのドメインは,約2億年前に融合した複製されたモノマーであることを示唆しています.
結論:
- アルテミアのポリメリックグロービン遺伝子は,複数の祖先のグロービン単体の融合によって進化した.
- 遺伝子融合は,無脊椎動物におけるポリメリックヘモグロビンの出現の可能性のあるメカニズムである.
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