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Updated: May 22, 2026

Identification and Characterization of Protein Glycosylation using Specific Endo- and Exoglycosidases
09:54

Identification and Characterization of Protein Glycosylation using Specific Endo- and Exoglycosidases

Published on: December 26, 2011

代替的なグリカン特異性を持つエンドグリコシダゼは,拡大されたグリコタンパク質の改造を可能にします

Jonathan J Goodfellow1, Kavitha Baruah, Keisuke Yamamoto

  • 1Department of Chemistry, University of Oxford, Chemistry Research Laboratory, Mansfield Road, Oxford OX1 3TA, UK.

Journal of the American Chemical Society
|May 4, 2012
PubMed
まとめ

新しい細菌のエンドグリコシダゼであるEndoSは,タンパク質グリカン改造の選択性を高めます. この酵素は,複雑なタイプのN結合グリカンを処理し,以前は難しい抗体グリコフォームに対応します.

関連する概念動画

Oligosaccharide Assembly01:24

Oligosaccharide Assembly

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Multiple sugar molecules that may or may...
Protein Glycosylation01:25

Protein Glycosylation

Glycosylation, the most common post-translational modification for proteins, serves diverse functions. Adding sugars to proteins makes the proteins more resistant to proteolytic digestion. Glycosylated proteins can act as markers and receptors to promote cell-cell adhesion. Additionally, they have many essential quality control functions in the cell, such as correct protein folding and facilitating transport of misfolded proteins to the cytosol, which can be degraded.
Glycosylation occurs in...
Proteoglycans01:05

Proteoglycans

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Protein Folding Quality Check in the RER01:29

Protein Folding Quality Check in the RER

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Protein Modifications in the RER01:26

Protein Modifications in the RER

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Broadly, these modifications can be categorized into four main categories — glycosylation, formation of disulfide bonds, assembly of protein subunits, and specific proteolytic cleavages like removal of signal sequences.
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
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