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関連する概念動画

Nuclear Export of mRNA02:31

Nuclear Export of mRNA

Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...
Nuclear Protein Sorting01:34

Nuclear Protein Sorting

Nuclear protein sorting is the selective trafficking of histones, polymerases, gene regulatory proteins into the nucleus and exporting RNAs and ribosomes to the cytosol. It is a tightly controlled process that regulates gene expression within a cell.
Proteins targeted to the nucleus carry nuclear localization signals or NLS recognized by import receptors in the cytosol. Similarly, proteins with nuclear export signals are recognized by export receptors. Import and export receptors are...
Nuclear Localization Signals and Import01:46

Nuclear Localization Signals and Import

Proteins targeted to the nucleus carry short stretches of amino acid sequences called the nuclear localization signal or NLS. Classical nuclear localization signals are of two types: monopartite and bipartite NLS. Monopartite classical NLS (cNLS) consists of a single cluster of 4-8 amino acids. Bipartite cNLS consists of two clusters of  2-3 amino acids and a 9-12 residue long proline-rich linker bridging the two clusters. Signal clusters are rich in positively charged amino acids such as...
Nuclear Export01:42

Nuclear Export

The nucleus restricts several proteins within and allows others to pass. The restricted proteins possess a nuclear retention sequence or NRS, anchoring them to the nuclear lamins and preventing their transport to the cytosol. The non-restricted proteins, after their synthesis, are transported to their site of action, such as the cytosol or other organelles, with the help of nuclear export signals or NES.
NES are of three types- the canonical 10-residue long leucine-rich signal and other...
Directionality of Nuclear Transport01:42

Directionality of Nuclear Transport

Ras-related nuclear protein or Ran is a small G protein that cycles between its GTP and GDP bound states. Ran specific regulators, a Ran GTPase Activating Protein or RanGAP present in the cytosol and a Ran guanine nucleotide exchange factor or RanGEF present inside the nucleus regulate GTP/GDP exchange. A high concentration of GTP inside the cells, in addition to this asymmetric distribution of  Ran-specific regulators, leads to a higher RanGTP concentration inside the nucleus. This...
Nuclear Export of mRNA02:31

Nuclear Export of mRNA

Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...

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関連する実験動画

Updated: May 7, 2026

Analysis of mRNA Nuclear Export Kinetics in Mammalian Cells by Microinjection
11:32

Analysis of mRNA Nuclear Export Kinetics in Mammalian Cells by Microinjection

Published on: December 4, 2010

RNPの輸出は,核エンベロープの芽生えによる.

Emily M Hatch1, Martin W Hetzer

  • 1Molecular and Cell Biology Laboratory, Salk Institute for Biological Studies, La Jolla, CA 92037, USA.

Cell
|May 15, 2012
PubMed
まとめ
この要約は機械生成です。

筋肉細胞は,新しいmRNA輸出経路を利用し,核毛孔複合体を回避する. リボ核タンパク質複合体は,神経筋の結合形成のために核膜を通過します.

さらに関連する動画

Small-scale Nuclear Extracts for Functional Assays of Gene-expression Machineries
08:05

Small-scale Nuclear Extracts for Functional Assays of Gene-expression Machineries

Published on: June 27, 2012

Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution
10:53

Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution

Published on: January 16, 2017

関連する実験動画

Last Updated: May 7, 2026

Analysis of mRNA Nuclear Export Kinetics in Mammalian Cells by Microinjection
11:32

Analysis of mRNA Nuclear Export Kinetics in Mammalian Cells by Microinjection

Published on: December 4, 2010

Small-scale Nuclear Extracts for Functional Assays of Gene-expression Machineries
08:05

Small-scale Nuclear Extracts for Functional Assays of Gene-expression Machineries

Published on: June 27, 2012

Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution
10:53

Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution

Published on: January 16, 2017

科学分野:

  • 分子生物学は分子生物学である.
  • 細胞生物学 細胞生物学
  • 神経科学は神経科学である.

背景:

  • 核孔複合体 (NPC) は,核から細胞質へのメッセンジャーRNA (mRNA) 輸出の確立された経路である.
  • mRNAの輸送メカニズムを理解することは,遺伝子発現の調節と細胞機能にとって極めて重要です.

研究 の 目的:

  • 特殊な細胞タイプにおける代替的なmRNAエクスポートメカニズムを調査する.
  • 核包膜の横断でリボヌクレオプロテイン複合体輸送の経路を特定する.

主な方法:

  • 筋肉細胞におけるmRNA輸送の生細胞イメージング.
  • 神経筋肉の交差点形成中の核封筒のダイナミクスの分析.
  • 輸出されたリボ核タンパク質複合体の生化学的特徴.

主要な成果:

  • 筋肉細胞におけるmRNAエクスポートのための非NPC経路の発見.
  • リボヌクレオプロテイン複合体が核膜と融合し,核膜を通過する証拠.
  • この経路を利用して,神経筋結節の組み立てに関与する特定の複合体の特定.

結論:

  • 核輸出は,核孔複合体によってのみ媒介されるものではない.
  • 新しい膜密輸メカニズムは,特定の細胞機能のためのmRNAの輸出を容易にする.
  • この経路は,神経筋肉の結合の発達と維持に不可欠です.