Jove
Visualize
お問い合わせ
JoVE
x logofacebook logolinkedin logoyoutube logo
JoVEについて
概要リーダーシップブログJoVEヘルプセンター
著者向け
出版プロセス編集委員会範囲と方針査読よくある質問投稿
図書館員向け
推薦の声購読アクセスリソース図書館諮問委員会よくある質問
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experimentsアーカイブ
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教員リソースセンター教員サイト
利用規約
プライバシーポリシー
ポリシー

関連する概念動画

Phase II Reactions: Glutathione Conjugation and Mercapturic Acid Formation01:22

Phase II Reactions: Glutathione Conjugation and Mercapturic Acid Formation

Glutathione, a tripeptide made up of glutamate, cysteine, and glycine, is a critical player in the detoxification of drugs and xenobiotics via a process known as glutathione conjugation or mercapturic acid formation. This phase II biotransformation reaction involves the covalent binding of glutathione to a drug or its metabolite, enhancing the compound's water solubility and enabling its excretion.
Several distinctive characteristics distinguish glutathione conjugation from other phase II...
Sulfur Assimilation01:20

Sulfur Assimilation

Sulfur is an essential element in biological systems, contributing to synthesizing key biomolecules, including amino acids such as cysteine and methionine, and cofactors such as coenzyme A and biotin. Microorganisms primarily assimilate sulfur as sulfate (SO₄²⁻) from the environment, which must undergo a series of biochemical transformations before it can be incorporated into cellular components. As sulfate is highly oxidized, it must undergo assimilatory sulfate reduction to become...
The Supercomplexes in the Crista Membrane01:41

The Supercomplexes in the Crista Membrane

The mitochondrial cristae membrane is the primary site for the oxidative phosphorylation (OXPHOS) process of energy conversion mediated through respiratory complexes I to V. These complexes have been widely studied for decades, and it has been proven that they form supramolecular structures called respiratory supercomplexes (SC). These higher-order complexes may be crucial in maintaining the biochemical structure and improving the physiological activity of the individual complexes while...
Electron Transport Chain: Complex I and II01:46

Electron Transport Chain: Complex I and II

The mitochondrial electron transport chain (ETC) is the main energy generation system in the eukaryotic cells. However, mitochondria also produce cytotoxic reactive oxygen species (ROS) due to the large electron flow during oxidative phosphorylation. While Complex I is one of the primary sources of superoxide radicals, ROS production by Complex II is uncommon and may only be observed in cancer cells with mutated complexes.
ROS generation is regulated and maintained at moderate levels necessary...
Electron Transport Chain: Complex III and IV01:43

Electron Transport Chain: Complex III and IV

During the electron transport chain, electrons from NADH and FADH2 are first transferred to complexes I and II, respectively. These two complexes then transfer the electrons to ubiquinol, which carries them further to complex III. Complex III passes the electrons across the intermembrane space to Cyt c, which carries them further to complex IV. Complex IV donates electrons to oxygen and reduces it to water. As electrons pass through complexes I, III, and IV, the energy released aids the pumping...
Complexation Equilibria: The Chelate Effect01:19

Complexation Equilibria: The Chelate Effect

In complexation reactions, metal atoms or cations interact with ligands to form donor-acceptor adducts called metal complexes. Ligands that bind through one donor site are monodentate, ligands with two donor sites are bidentate, and those with more than two donor sites are polydentate ligands. For example, ethylene diamine is a bidentate ligand that binds through two nitrogen donor atoms, forming a five-membered ring. EDTA is a polydentate ligand that binds through four oxygen and two nitrogen...

こちらも読む

関連記事

共著者、ジャーナル、引用グラフによってこの研究に関連する記事。

並び替え
Same author

Structural and sequence basis for substrate selection in the cellular trafficking of Fe-S clusters, hemes and glutathione-complexed metals through membrane transporters.

Journal of inorganic biochemistry·2025
Same author

Understanding the Thermodynamics of Magnesium Binding to RNA Structural Motifs.

Life (Basel, Switzerland)·2024
Same author

Influence of the Weak Nuclear Force on Metal-Promoted Autocatalytic Strecker Synthesis of Amino Acids: Formation of a Chiral Pool of Precursors for Prebiotic Peptide and Protein Synthesis.

Life (Basel, Switzerland)·2024
Same author

Bidirectional Catalysis Disintegration and Mineral Polymerization via Endogenous Iron(III) from Iron-Rich Sludge in Synergy with Waste Incineration Fly Ash.

ACS omega·2023
Same author

Association of tirofiban with improvement of functional outcomes of direct thrombectomy for acute anterior circulation occlusion: a retrospective, nonrandomized, multicenter, real-world study.

Neurosurgical focus·2023
Same author

Ethyl Acetate Fraction from Hedyotis Diffusa Plus Scutellaria Barbata Inhibits the Progression of Breast Cancer <i>via</i> Targeting LMO1 and AKT/Mtor Signaling Pathway.

Combinatorial chemistry & high throughput screening·2023

関連する実験動画

Updated: May 21, 2026

Rapid Quantification of Oxidized and Reduced Forms of Glutathione Using Ortho -phthalaldehyde in Cultured Mammalian Cells In Vitro
03:35

Rapid Quantification of Oxidized and Reduced Forms of Glutathione Using Ortho -phthalaldehyde in Cultured Mammalian Cells In Vitro

Published on: June 28, 2024

グルタチオンの複合体を持つFe-Sセンター.

Wenbin Qi1, Jingwei Li, C Y Chain

  • 1Ohio State Biochemistry Program, The Ohio State University, 100 West 18th Avenue, Columbus, Ohio 43210, USA.

Journal of the American Chemical Society
|June 13, 2012
PubMed
まとめ
この要約は機械生成です。

グルタチオンは単独で鉄硫黄のクラスタを安定させることができます. この発見は,グルタチオンがグルタレドキシンとは独立して,細胞の鉄硫黄クラスター生物合成における新たな役割を明らかにしている.

さらに関連する動画

Resin-Assisted Capture Coupled with Isobaric Tandem Mass Tag Labeling for Multiplexed Quantification of Protein Thiol Oxidation
07:16

Resin-Assisted Capture Coupled with Isobaric Tandem Mass Tag Labeling for Multiplexed Quantification of Protein Thiol Oxidation

Published on: June 21, 2021

The Cell-based L-Glutathione Protection Assays to Study Endocytosis and Recycling of Plasma Membrane Proteins
09:22

The Cell-based L-Glutathione Protection Assays to Study Endocytosis and Recycling of Plasma Membrane Proteins

Published on: December 13, 2013

関連する実験動画

Last Updated: May 21, 2026

Rapid Quantification of Oxidized and Reduced Forms of Glutathione Using Ortho -phthalaldehyde in Cultured Mammalian Cells In Vitro
03:35

Rapid Quantification of Oxidized and Reduced Forms of Glutathione Using Ortho -phthalaldehyde in Cultured Mammalian Cells In Vitro

Published on: June 28, 2024

Resin-Assisted Capture Coupled with Isobaric Tandem Mass Tag Labeling for Multiplexed Quantification of Protein Thiol Oxidation
07:16

Resin-Assisted Capture Coupled with Isobaric Tandem Mass Tag Labeling for Multiplexed Quantification of Protein Thiol Oxidation

Published on: June 21, 2021

The Cell-based L-Glutathione Protection Assays to Study Endocytosis and Recycling of Plasma Membrane Proteins
09:22

The Cell-based L-Glutathione Protection Assays to Study Endocytosis and Recycling of Plasma Membrane Proteins

Published on: December 13, 2013

科学分野:

  • バイオケミストリー バイオケミストリー
  • 分子生物学は分子生物学である.
  • バイオ・オーガニック化学 バイオ・オーガニック化学

背景:

  • グルタチオン (γ-グルタミル-システニル-グリシン,GSH) は,細胞内の高濃度で発見された重要なチオールを含むペプチドです.
  • 最近の研究によると,グルタレドキシン (Grx) は,リガンドとしてグルタチオンを用いて鉄硫黄のクラスター形成を媒介する.
  • 鉄と硫黄のクラスター生物合成におけるグルタチオンの正確な役割は,依然として活発な研究分野です.

研究 の 目的:

  • グルタチオンと鉄硫黄のクラスターの間の直接的な相互作用を調査するために.
  • グルタチオンに結合した鉄硫黄複合体の構造と安定性を特徴付けるため.
  • グルタチオンを含む鉄硫黄のクラスター形成のメカニズムを解明する.

主な方法:

  • 複合体を特徴付けるために,光学分析 (光学,リドックス,モースバウアー,NMR) を行う.
  • Fe-Sアセンブリタンパク質ISUを用いたインビロアッセイ.
  • グルタチオンの存在下での鉄と硫化物イオンの定位.

主要な成果:

  • グルタチオンは単独で,生理学的条件下で[Fe(2) S(2) ]クラスタを調整し安定させることができます.
  • その結果生成した複合体[Fe(2) S(2) ](GS) ((4) は,異なるスペクトル学的性質を示している.
  • ISUタンパク質は[Fe2S2GS4]の形成を触媒化し,その鉄硫黄核をApo ISUと自由グルタチオンと逆転的に交換することができる.

結論:

  • グルタチオンは,鉄硫黄のクラスターの安定化に直接参加します.
  • この相互作用は,細胞の鉄と硫黄のクラスター生物合成のための新しい経路を提供します.
  • この発見は,細胞プロセスにおけるグルタチオンの多面的な役割についての理解を広げています.