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Native Cell Membrane Nanoparticles System for Membrane Protein-Protein Interaction Analysis07:31

Native Cell Membrane Nanoparticles System for Membrane Protein-Protein Interaction Analysis

6.5K
Presented here is a protocol for the determination of oligomeric state of membrane proteins that utilizes a native cell membrane nanoparticle system in conjunction with electron...
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From Constructs to Crystals – Towards Structure Determination of β-barrel Outer Membrane Proteins09:55

From Constructs to Crystals – Towards Structure Determination of β-barrel Outer Membrane Proteins

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β-barrel outer membrane proteins (OMPs) serve many functions within the outer membranes of Gram-negative bacteria, mitochondria, and chloroplasts. Here, we hope to alleviate a known bottleneck in structural studies by presenting protocols for the production of β-barrel OMPs in sufficient quantities for structure determination by X-ray crystallography or NMR...
14.0K
Green Fluorescent Protein-based Expression Screening of Membrane Proteins in Escherichia coli08:46

Green Fluorescent Protein-based Expression Screening of Membrane Proteins in Escherichia coli

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A streamlined approach to screening for the expression of recombinant membrane proteins in Escherichia coli based on fusion to green fluorescent protein is...
33.6K
A Protocol for Computer-Based Protein Structure and Function Prediction16:41

A Protocol for Computer-Based Protein Structure and Function Prediction

69.7K
Guidelines for computer based structural and functional characterization of protein using the I-TASSER pipeline is described. Starting from query protein sequence, 3D models are generated using multiple threading alignments and iterative structural assembly simulations. Functional inferences are thereafter drawn based on matches to proteins with known structure and...
69.7K
Crystallizing Membrane Proteins for Structure Determination using Lipidic Mesophases22:00

Crystallizing Membrane Proteins for Structure Determination using Lipidic Mesophases

30.6K
Herein is described the procedure implemented in the Caffrey Membrane Structural and Functional Biology Group to set up manually crystallization trials of membrane proteins in lipidic...
30.6K
Protein and Protein Structure02:15

Protein and Protein Structure

87.0K
Proteins are one of the most abundant organic molecules in living systems and have the most diverse range of functions of all macromolecules. Proteins may be structural, regulatory, contractile, or protective. They may serve in transport, storage, or membranes; or they may be toxins or enzymes. Their structures, like their functions, vary greatly. They are all, however, amino acid polymers arranged in a linear sequence.
A protein's shape is critical to its function. For example, an enzyme...
87.0K

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関連する実験動画

Updated: Jan 20, 2026

Native Cell Membrane Nanoparticles System for Membrane Protein-Protein Interaction Analysis
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Native Cell Membrane Nanoparticles System for Membrane Protein-Protein Interaction Analysis

Published on: July 16, 2020

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探査のための膜タンパク質構造の予測

Nick V Grishin1

  • 1Howard Hughes Medical Institute, Department of Biophysics, University of Texas Southwestern Medical Center, Dallas, TX 75390-9050, USA. grishin@chop.swmed.edu

Cell
|June 26, 2012
PubMed
まとめ
この要約は機械生成です。

ホップフ et al. によるものです. 配列アライメントを用いた11のトランスメブランタンパク質ファミリーの新規のタンパク質構造モデルを開発した. これらのモデルは,将来の実験的検証と構造生物学の進歩に希望を示しています.

科学分野:

  • 構造生物学 構造生物学とは
  • 計算生物学とは,計算生物学である.
  • ゲノミクスゲノミクスとは

背景:

  • タンパク質の構造を予測することは,生物学的機能を理解するために極めて重要です.
  • トランスメブランタンパク質は細胞プロセスにおいて重要な役割を果たしますが,構造的に研究することは困難です.
  • 配列の並べ替えは,アミノ酸残基間の空間的関係を推論するための貴重なリソースを提供します.

さらに関連する動画

A Protocol for Computer-Based Protein Structure and Function Prediction
16:41

A Protocol for Computer-Based Protein Structure and Function Prediction

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Crystallizing Membrane Proteins for Structure Determination using Lipidic Mesophases
22:00

Crystallizing Membrane Proteins for Structure Determination using Lipidic Mesophases

Published on: November 21, 2010

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関連する実験動画

Last Updated: Jan 20, 2026

Native Cell Membrane Nanoparticles System for Membrane Protein-Protein Interaction Analysis
07:31

Native Cell Membrane Nanoparticles System for Membrane Protein-Protein Interaction Analysis

Published on: July 16, 2020

6.5K
A Protocol for Computer-Based Protein Structure and Function Prediction
16:41

A Protocol for Computer-Based Protein Structure and Function Prediction

Published on: November 3, 2011

69.7K
Crystallizing Membrane Proteins for Structure Determination using Lipidic Mesophases
22:00

Crystallizing Membrane Proteins for Structure Determination using Lipidic Mesophases

Published on: November 21, 2010

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