マウスのゲノムにおけるシス調節配列の地図

Yin Shen¹, Feng Yue, David F McCleary

¹Ludwig Institute for Cancer Research, 9500 Gilman Drive, La Jolla, California 92093-0653, USA.

Nature

|July 6, 2012

まとめ

研究者らは,マウスの19の組織で約30万のシス調節配列をマッピングした. マウスゲノムのこの重要な注釈は,ヒトゲノム機能と遺伝子調節を理解するのに役立ちます.

科学分野:

ゲノミクスゲノミクスとは
分子生物学は分子生物学である.
バイオインフォマティックス

背景:

マウスは,生物医学研究における重要な哺乳類モデル生物です.
マウスのゲノムは,ヒトのゲノムと高い保全率を共有しており,そのアノテーションはヒト生物学にとって貴重なものである.
マウスのゲノムにおける機能的およびシス調節配列は,主に注釈されていないままである.

研究の目的:

マウスのゲノムにおけるシス調節配列を包括的にマッピングする.
組織特異的な増強剤とそのトランスクリプションを調節する因子を特定する.
マウスのゲノム内の規制要素の組織を理解する.

主な方法:

19種類の異なるマウス組織と細胞タイプでクロマチン免疫プレシピテーションシーケンシング (ChIP-Seq) を利用しました.
保存された非コーディング配列を特定するために比較ゲノミクス原理を適用した.
既存のゲノムアノテーションと統合されたChIP-Seqデータ.

主要な成果:

約30万のマウスのシス調節配列のマップを作成し,マウスゲノムの11%をカバーしました.
保存された非コード配列の70%以上で注釈されている.
特定された組織特異的増強剤と潜在的転写因子を特定し,調整された規制領域を明らかにした.

結論:

この研究は,哺乳類のゲノム内の機能的要素を注釈するための貴重なリソースを提供します.
この発見は,組織特異的な遺伝子発現機構の理解を深める.
総合的な地図は,マウスとヒトのゲノムに関する将来の研究を促進します.

さらに関連する動画

07:50

Chromatin Immunoprecipitation of Murine Brown Adipose Tissue

Published on: November 21, 2018

7.7K

11:41

Mapping Mammalian 3D Genome Interactions with Micro-C-XL

Published on: November 3, 2023

4.2K

関連する概念動画

02:02

Cis-regulatory Sequences

Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...

02:13

Cooperative Binding of Transcription Regulators

Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome. Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form dimers that...

02:04

Co-activators and Co-repressors

Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...

02:23

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...

02:51

Multi-species Conserved Sequences

Next-generation sequencing technologies have created large genomic databases of a variety of animals and plants. Ever since the human genome project was completed, scientists studied the genome of primates, mammals, and other phylogenetically distant living beings. Such large-scale studies have provided new insights into the evolutionary relationship between organisms.
Although the genome of each species varies greatly from each other, a few sequences are highly conserved. Such conserved DNA...