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Updated: May 20, 2026

Combining Non-reducing SDS-PAGE Analysis and Chemical Crosslinking to Detect Multimeric Complexes Stabilized by Disulfide Linkages in Mammalian Cells in Culture
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Combining Non-reducing SDS-PAGE Analysis and Chemical Crosslinking to Detect Multimeric Complexes Stabilized by Disulfide Linkages in Mammalian Cells in Culture

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クイエスチン硫ヒドリル酸化酵素のダイナミック・ディスルフィード・リレー

Assaf Alon1, Iris Grossman, Yair Gat

  • 1Department of Structural Biology, Weizmann Institute of Science, Rehovot 76100, Israel.

Nature
|July 18, 2012
PubMed
まとめ

キエスチン硫ヒドリル酸化酵素 (QSOX) は,ドメインの回転を使用して,遠隔の活性部位間のディスルフィードを転送します. この構造的な柔軟性は,その触媒機構を説明し,多領域酵素設計の洞察を提供します.

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