Jove
Visualize
お問い合わせ
JoVE
x logofacebook logolinkedin logoyoutube logo
JoVEについて
概要リーダーシップブログJoVEヘルプセンター
著者向け
出版プロセス編集委員会範囲と方針査読よくある質問投稿
図書館員向け
推薦の声購読アクセスリソース図書館諮問委員会よくある質問
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experimentsアーカイブ
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教員リソースセンター教員サイト
利用規約
プライバシーポリシー
ポリシー

関連する概念動画

siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
piRNA - Piwi-interacting RNAs02:57

piRNA - Piwi-interacting RNAs

PIWI-interacting RNAs, or piRNAs, are the most abundant short non-coding RNAs. More than 20,000 genes have been found in humans that code for piRNAs while only 2000 genes have been found for miRNAs. piRNAs can act at the transcriptional and post-transcriptional levels and have a vital role in silencing transposable elements present in germ cells. They are also involved in epigenetic silencing and activation. Previously, they were thought to function only in germ cells but new evidence suggests...
lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA (lncRNA)...

こちらも読む

関連記事

共著者、ジャーナル、引用グラフによってこの研究に関連する記事。

並び替え
Same author

Addressing the needs of nano-rare patients: the n-Lorem experience.

Nucleic acids research·2026
Same author

Antisense Reduction of Mutant COMP Reduces Growth Plate Chondrocyte Pathology.

Molecular therapy : the journal of the American Society of Gene Therapy·2026
Same author

Distribution of divalent small interfering RNA into neurons of sensory ganglia produces selective, durable knockdown of Nav1.7 and strong analgesia.

Pain·2026
Same author

Enhanced splicing modulation by NMA-modified antisense oligonucleotides.

Nucleic acids research·2026
Same author

Divalent siRNA for prion disease.

Nucleic acids research·2026
Same author

Phosphorothioate antisense oligonucleotide induced innate immune activation is attenuated by tryptophan oxidation products.

Nucleic acids research·2026

関連する実験動画

Updated: May 19, 2026

Rearing and Double-stranded RNA-mediated Gene Knockdown in the Hide Beetle, Dermestes maculatus
09:57

Rearing and Double-stranded RNA-mediated Gene Knockdown in the Hide Beetle, Dermestes maculatus

Published on: December 28, 2016

単一鎖siRNAは,動物におけるRNAiを活性化する.

Walt F Lima1, Thazha P Prakash, Heather M Murray

  • 1Core Antisense Research, Isis Pharmaceuticals Inc., Carlsbad, CA 92010, USA.

Cell
|September 4, 2012
PubMed
まとめ

強力な単一鎖siRNAs (ss-siRNAs) は,複雑な配送なしに vivo で遺伝子を沈黙させることができます. この発見はRNA干渉 (RNAi) 治療法を簡素化し,RNAiメカニズムに関する新しい洞察を提供します.

さらに関連する動画

Cell Based Assays of SINEUP Non-coding RNAs That Can Specifically Enhance mRNA Translation
10:21

Cell Based Assays of SINEUP Non-coding RNAs That Can Specifically Enhance mRNA Translation

Published on: February 1, 2019

関連する実験動画

Last Updated: May 19, 2026

Rearing and Double-stranded RNA-mediated Gene Knockdown in the Hide Beetle, Dermestes maculatus
09:57

Rearing and Double-stranded RNA-mediated Gene Knockdown in the Hide Beetle, Dermestes maculatus

Published on: December 28, 2016

Cell Based Assays of SINEUP Non-coding RNAs That Can Specifically Enhance mRNA Translation
10:21

Cell Based Assays of SINEUP Non-coding RNAs That Can Specifically Enhance mRNA Translation

Published on: February 1, 2019

科学分野:

  • バイオケミストリー バイオケミストリー
  • 分子生物学は分子生物学である.
  • 薬理学 薬理学とは

背景:

  • 小型の干渉RNA (siRNA) の治療応用は,複雑な配送システムの必要性によって妨げられています.
  • 強力な単一鎖RNAを特定することで,RNAベースの薬の開発を簡素化することができます.

研究 の 目的:

  • 脂質製剤なしで遺伝子サイレンスを行う単一鎖siRNAs (ss-siRNAs) を vivo で開発し,特徴づけること.
  • 強化された効能,安定性,および薬理学的な性質のためのss-siRNAsの構造-活性関係を調査する.

主な方法:

  • 単一鎖RNAの繰り返し設計と化学的改変.
  • 化学的変化とアルゴナウト2 (AGO2) の活性,細胞の効能,核酵素の安定性,および薬理学を相関させる構造-活性関係の研究.
  • 遺伝子サイレンシングの有効性に関するss-siRNAsのインビボ試験.

主要な成果:

  • 脂質ベースの配達なしで動物における遺伝子発現を沈黙させる効果的なss-siRNAsを特定しました.
  • 乗客鎖は,RNA干渉 (RNAi) 経路による強力な遺伝子静止に不可欠ではないことが実証されました.
  • ss-siRNAの活性 in vivoは5'フォスファートを必要とし,安定した5'-(E) -ビニルフォスフォナート (5'-VP) アナログの開発につながることが確認されました.

結論:

  • 強力なss-siRNAsは,複雑な配送要件を回避して,RNAi療法への簡素化されたアプローチを表しています.
  • この発見は,RNAiのメカニズムに関する新しい視点を提供し,ガイドストランドと5'-リン酸塩の重要な役割を強調しています.
  • この研究は,RNAiベースの薬理学的薬剤の開発の選択肢を拡大します.