関連する実験動画

Updated: May 19, 2026

A G-quadruplex DNA-affinity Approach for Purification of Enzymatically Active G4 Resolvase1
11:25

A G-quadruplex DNA-affinity Approach for Purification of Enzymatically Active G4 Resolvase1

Published on: March 18, 2017

DEAD-boxヘリケーズMss116pによるRNA-デュプレックス認識と解き放出の構造的基礎

Anna L Mallam1, Mark Del Campo, Benjamin Gilman

  • 1Institute for Cellular and Molecular Biology, Department of Chemistry and Biochemistry, University of Texas at Austin, Austin, Texas 78712, USA.

Nature
|September 4, 2012
PubMed
まとめ

DEAD-boxタンパク質は,モジュールドメインを含むユニークなメカニズムを使用してRNAを解き放つ. ドメイン1はATPを結合し,ドメイン2はRNA複製を認識し,特定の解き放出を可能にします.

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Single-Strand DNA Binding Proteins

For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...
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The DNA Replication Fork01:02

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Overview
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Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...