人間の転写因子規制ネットワークの回路とダイナミクス
Shane Neph1, Andrew B Stergachis, Alex Reynolds
1Department of Genome Sciences, University of Washington, Seattle, WA 98195, USA.
Cell
|September 11, 2012
まとめ
人間の転写因子 (TF) ネットワークは,細胞特異性が高く,多様な細胞タイプが共通の規制アーキテクチャに収束しています. この研究は,これらのネットワークをマッピングして,細胞のアイデンティティと機能の原理を明らかにします.
科学分野:
- ゲノミクスゲノミクスとは
- システム生物学 システム生物学
- 分子生物学は分子生物学である.
背景:
- 細胞のアイデンティティと機能は,配列特異の転写因子 (TF) の複雑な規制ネットワークによって支配されます.
- これらのTFネットワークのダイナミクスとアーキテクチャを理解することは,細胞プロセスの解読に不可欠です.
研究 の 目的:
- ゲノム全体のデータを用いて広範なヒトTF規制ネットワークを組み立てる.
- これらのTF接続の細胞型特異性と動的性質を分析する.
- 人間TFの規制ネットワークの基礎となる組織原理を明らかにする.
主な方法:
- in vivo DNaseI 足跡の全ゲノムマップを使用しました.
- 475のシーケンス固有のTFのコアヒューマン規制ネットワークを組み立てました.
- 41の多様なヒトの細胞・組織タイプにおけるTFネットワークのダイナミクスを分析した.
主要な成果:
- 人間のTFネットワークは,特定のTFコホートによって駆動される高い細胞選択性を示す.
- ネットワークをセル選択的に調節する広く表現されたTFを特定した.
- ニューロンネットワークに似ている,多様な細胞タイプに共通するネットワークアーキテクチャを発見した.
結論:
- 人間のTFネットワークはダイナミックで,細胞選択性があり,保存されたアーキテクチャに収束します.
- 細胞のアイデンティティを制御する上で,以前未知のTFの役割を明らかにする.
- 人間TFの規制回路とその組織原理の包括的な地図を提供します.
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