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2つの長いノンコーディングRNAのトランスクリプションは,芽生えた酵母菌におけるゲメトゲネシスの交配型の制御を媒介する
Folkert J van Werven1, Gregor Neuert, Natalie Hendrick
1David H. Koch Institute for Integrative Cancer Research and Howard Hughes Medical Institute, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
Cell
|September 11, 2012
まとめ
2つの長いノンコーディングRNAs (lncRNAs) は,IME1遺伝子を調節することによって,酵母ゲメトゲネシスを制御します. 彼らの転写はハプロイドの胞子化を阻害しますが,細胞運命を決定するディプロイド誘導には不可欠です.
科学分野:
- 分子生物学は分子生物学である.
- 遺伝学 遺伝学とは
- 細胞生物学 細胞生物学
背景:
- 性繁殖はゲメトゲネシス (ゲメトゲネシス) に依存しており,これは細胞運命を決定する重要な決定です.
- 酵母菌 (Saccharomyces cerevisiae) では,二倍体細胞 (MATa/α) だけが胞子化し,ハプロイド細胞 (MATaまたはMATα) は発生しない.
- 交尾型の胞子形成の制御は,重要な規制プロセスである.
研究 の 目的:
- ゲメトゲネシスの交配型制御におけるロングノンコーディングRNA (lncRNA) の役割を調査する.
- lncRNAsが酵母菌の胞子を調節する分子メカニズムを解明する.
主な方法:
- ハプロイドおよび二プロイド酵母細胞におけるIncRNA転写の分析.
- IME1とIME4の遺伝子調節に対するlncRNA発現の影響を調査する.
- IME1プロモーターにおけるヒストンの改変を評価するためのクロマチン免疫プレシピテーション.
主要な成果:
- 2つの特定のlncRNAs,IRT1とアンチセンセスのトランスクリプトは,交配型制御の媒介者として特定されました.
- ハプロイドのIRT1転写は,抑圧的なクロマチン変容体 (Set2,Set3複合体) を採用することによって,中央ゲメトゲネシス誘導体IME1を阻害する.
- これらのIncRNAをハプロイドで破壊すると,胞子形成が可能になり,二倍体ではその存在が胞子形成を無効化する.
結論:
- 特定のlncRNAsの転写は,酵母におけるゲメトゲネシスの交配型依存的調節を統制する.
- lncRNAは重要な調節体として機能し,性繁殖のための正しい細胞運命を決定します.
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