マウスの胚の結節にあるシリアは,Pkd2経由で左右の決定のために,液体の流れを感知する
Satoko Yoshiba1, Hidetaka Shiratori, Ivana Y Kuo
1Developmental Genetics Group, Graduate School of Frontier Biosciences, Osaka University, 1-3 Yamada-oka, Suita, 565-0871 Osaka, Japan.
Science (New York, N.Y.)
|September 18, 2012
まとめ
マウスの胚冠細胞のポリシスティン-2 (Pkd2) は,左-右対称性を破るために不可欠な結節液の流れを感知します. Pkd2のシリアの局所化は,このフローセンシングメカニズムにとって非常に重要です.
科学分野:
- 発達生物学 発達生物学について
- 胚形成とは,胚の形成である.
- 細胞生物学 細胞生物学
背景:
- マウスの胚における左-右 (L-R) 非対称性の破裂は,一方的な流体流れに依存しています.
- 胚がこの結節の流れを感知するメカニズムは,ほとんど不明のままである.
研究 の 目的:
- ネズミの胚で結節液の流れを感知する分子成分を特定する.
- L-R対称性破裂におけるポリシスティン-2 (Pkd2) の役割を明らかにする.
主な方法:
- 機能的なPkd2またはシリア (Kif3a変異体) が欠けている変異性マウス胚を使用した.
- 冠細胞内のPkd2の局所性を調べました.
- 遺伝子組み換え胚における人工結節流への反応を評価した.
主要な成果:
- ポリシスティン-2 (Pkd2) は,ノダルの流れを感知するために,ペリノダルの冠細胞に特に必要とされています.
- Pkd2の状局所化は,適切なL-Rパターンの形成に不可欠である.
- クラウン細胞のプライマリシリアの回復は,Kif3a変異胚のフロー応答を救出しました.
結論:
- 結節の流れは,結節の端にある冠細胞のシリアによって感知されます.
- この感知メカニズムは,Ca2+チャネルであるポリシスティン-2 (Pkd2) に依存しています.
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