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Master Transcription Regulators02:23

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
Canonical Wnt Signaling Pathway02:54

Canonical Wnt Signaling Pathway

The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which results in tumor...
Canonical Wnt Signaling Pathway02:54

Canonical Wnt Signaling Pathway

The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which results in tumor...
T Cell Activation and Clonal Selection01:22

T Cell Activation and Clonal Selection

T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
T Cell Types and Functions01:24

T Cell Types and Functions

When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
B Cell Activation and Differentiation01:24

B Cell Activation and Differentiation

The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...

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Mouse Naïve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets
07:12

Mouse Naïve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets

Published on: April 16, 2015

Th17 細胞の仕様に関する検証された規制ネットワーク.

Maria Ciofani1, Aviv Madar, Carolina Galan

  • 1Molecular Pathogenesis Program, The Kimmel Center for Biology and Medicine of the Skirball Institute, New York University School of Medicine, New York, NY 10016, USA.

Cell
|October 2, 2012
PubMed
まとめ
この要約は機械生成です。

研究者は,Tヘルパー17 (Th17) 細胞の分化を制御する規制ネットワークをマッピングしました. これは,RORγtやBATFのような重要な転写因子を明らかにし,炎症性疾患に対する新しい治療標的を提供している.

さらに関連する動画

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Retroviral CRISPR/Cas9-Mediated Gene Targeting for the Study of Th17 Differentiation in Vitro
12:08

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関連する実験動画

Last Updated: May 10, 2026

Mouse Naïve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets
07:12

Mouse Naïve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets

Published on: April 16, 2015

In Vitro Differentiation of Naive CD4+ T Cells into Pathogenic Th17 Cells in Mouse
07:46

In Vitro Differentiation of Naive CD4+ T Cells into Pathogenic Th17 Cells in Mouse

Published on: October 25, 2024

Retroviral CRISPR/Cas9-Mediated Gene Targeting for the Study of Th17 Differentiation in Vitro
12:08

Retroviral CRISPR/Cas9-Mediated Gene Targeting for the Study of Th17 Differentiation in Vitro

Published on: November 15, 2024

科学分野:

  • 免疫学 免疫学とは
  • 分子生物学は分子生物学である.
  • システム生物学 システム生物学

背景:

  • Tヘルパー17 (Th17) 細胞は粘膜免疫に不可欠ですが,炎症性疾患にも関与しています.
  • Th17細胞の分化は,RORγtを含む転写因子 (TFs) の複雑な相互作用によって指揮されています.

研究 の 目的:

  • Th17細胞の微分化を統制するグローバルな転写制御ネットワークの描写を図る.
  • 新規の規制当局を特定し,主要なTFの階層的な役割を理解する.

主な方法:

  • ゲノム全体のTF占有率,TF変異体表現プロファイリング,およびタイムシリーズ表現データを組み合わせた反復的なシステム生物学アプローチを使用しました.
  • Th17 規制ネットワークの予測モデルを構築し,検証するために複数のデータセットを統合しました.

主要な成果:

  • 初期クロマチンのアクセシビリティとトランスクリプションの開始に不可欠なBATFとIRF4を特定しました.
  • RORγtがTh17の転写プログラムをグローバルに調節する焦点的,決定的な役割を果たしていることを実証した.
  • Fosl2を含む新しいTh17調節体を発見し,細胞の可塑性におけるその役割を強調した.

結論:

  • この研究は,Th17転写制御ネットワークの包括的な地図を提示しています.
  • 検証された予測モデルは,TFの協力と階層的なコントロールの洞察を提供します.
  • 特定されたレギュレータとネットワークダイナミクスは,Th17媒介の炎症性疾患に対する新しい治療戦略の開発のための基盤を提供します.