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関連する概念動画

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
RNA Editing02:23

RNA Editing

RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the addition of a...

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関連する実験動画

Updated: May 7, 2026

Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome
07:23

Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome

Published on: June 15, 2016

人間のRNAメチルトランスフェラーゼBCDIN3Dは,マイクロRNAの処理を調節する.

Blerta Xhemalce1, Samuel C Robson, Tony Kouzarides

  • 1Wellcome Trust/Cancer Research UK Gurdon Institute, The Henry Wellcome Building of Cancer and Developmental Biology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QN, UK.

Cell
|October 16, 2012
PubMed
まとめ

新しいRNAメチルトランスフェラーゼ,BCDIN3DがO-メチラートマイクロRNA (miRNA) 前駆体で発見され,その成熟を阻害しました. この発見は,がん細胞の成長に影響を与える新しいmiRNAメチル化経路を明らかにしています.

科学分野:

  • 分子生物学は分子生物学である.
  • バイオケミストリー バイオケミストリー
  • がん研究 がん研究

背景:

  • マイクロRNA (miRNA) は生物学的プロセスの重要な調節体であり,その調節不良は癌と関連しています.
  • miRNAバイオゲネシスには,ドロシャとディッサーによる連続処理があり,機能に不可欠な5'-モノフォスファート末端を生成します.
  • プリミRNAの5'-モノホスファートのDicerの認識は,正確なmiRNA生成に不可欠です.

研究 の 目的:

  • miRNAバイオゲネシスの新しいレギュレータを特定する.
  • miRNA処理におけるRNAメチルトランスファーゼの役割を調査する.
  • がんにおけるmiRNA調節の潜在的な関与を探求する.

主な方法:

  • BCDIN3Dの活性を研究するためのインビトロおよびインビボ生化学アッセイ.
  • BCDIN3D操作後のプレミRNAおよび成熟ミRNAレベルの分析.
  • BCDIN3Dの枯渇による乳がん細胞フェノタイプの評価.

主要な成果:

  • RNA-メチルトランスフェラーゼであるBCDIN3Dは,プリミRNAの5'-モノフォスファートのO-メチレーターとして特定されました.

さらに関連する動画

Genome-wide Analysis of HDAC Inhibitor-mediated Modulation of microRNAs and mRNAs in B Cells Induced to Undergo Class-switch DNA Recombination and Plasma Cell Differentiation
11:06

Genome-wide Analysis of HDAC Inhibitor-mediated Modulation of microRNAs and mRNAs in B Cells Induced to Undergo Class-switch DNA Recombination and Plasma Cell Differentiation

Published on: September 20, 2017

Antibody-Free Assay for RNA Methyltransferase Activity Analysis
08:31

Antibody-Free Assay for RNA Methyltransferase Activity Analysis

Published on: July 9, 2019

関連する実験動画

Last Updated: May 7, 2026

Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome
07:23

Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome

Published on: June 15, 2016

Genome-wide Analysis of HDAC Inhibitor-mediated Modulation of microRNAs and mRNAs in B Cells Induced to Undergo Class-switch DNA Recombination and Plasma Cell Differentiation
11:06

Genome-wide Analysis of HDAC Inhibitor-mediated Modulation of microRNAs and mRNAs in B Cells Induced to Undergo Class-switch DNA Recombination and Plasma Cell Differentiation

Published on: September 20, 2017

Antibody-Free Assay for RNA Methyltransferase Activity Analysis
08:31

Antibody-Free Assay for RNA Methyltransferase Activity Analysis

Published on: July 9, 2019

  • BCDIN3Dは,dicer.による処理を減らすために,pre-miR-145のフォスフォ・ディメチラートを示した.
  • 乳がん細胞におけるBCDIN3Dの枯渇により,成熟したmiR-145レベルが上昇し,腫瘍発生性現象型が抑制されました.

    • 結論:

    • BCDIN3Dは,プリミRNAのOメチル化によってmiRNAの成熟を否定的に制御する.
    • この新しいmiRNAメチル化経路は,Dicer依存処理を逆転させ,miR-145および潜在的に他のmiRNAに影響を与える.
    • この発見は,がん生物学に関連する miRNA 調節の新しいメカニズムを示唆しています.