ネズミのmtDNAのヘテロプラズミーは遺伝的に不安定であり,行動や認知の変化に起因する
Mark S Sharpley1,2,3, Christine Marciniak2,3, Kristin Eckel-Mahan3,4
1Center for Mitochondrial and Epigenomic Medicine, the Children's Hospital of Philadelphia and the Department of Pathology and Laboratory Medicine, University of Pennsylvania, Colket Translational Research Building, Philadelphia, PA 19104-4302, USA.
Cell
|October 16, 2012
まとめ
マウスにおけるミトコンドリアDNA (mtDNA) の混合は,遺伝的不安定性と有害な生理学的効果を引き起こし,なぜ母親からの遺伝が動物に共通するのか示唆しています.
科学分野:
- 遺伝学 遺伝学とは
- ミトコンドリア生物学
- 動物生理学 動物生理学
背景:
- ミトコンドリアDNA (mtDNA) の母性継承は動物に共通していますが,その根本的な理由は十分に理解されていません.
- 単親の継承はmtDNAの遺伝的一貫性を保証するが,混合mtDNA (異質プラズミア) の影響はほとんど未知のものである.
研究 の 目的:
- 異なる株の混合 mtDNA を持つマウスを生み出すことにより,厳格な母性遺伝を優先する効果を調査する.
- mtDNAヘテロプラズミーの遺伝的安定性と生理学的結果を分析する.
主な方法:
- 核背景C57BL/6Jで,NZBと129S6株からヘテロプラズミックミトコンドリアDNA (mtDNA) を生成したマウス.
- 2つのmtDNAタイプの分離を母親の世代にわたって追跡した.
- ヘテロプラズマ・マウスとホモプラズマ・マウスの相似フェノタイプ.
主要な成果:
- 分離中にNZB mtDNAの好ましい減少が観察され,ヘテロプラズマおよびホモプラズマの子孫が生まれた.
- 混合NZB-129 mtDNAを持つマウスは,活動,食物摂取,呼吸器交換比率の低下を示した.
- ヘテロプラズマのマウスは,ホモプラズマのマウスと比較して,ストレス反応と認知障害が顕著に示されました.
結論:
- 異なる,しかし正常なマウスのmtDNAを混ぜ合わせると,遺伝的不安定性と有害な生理学的結果が生じます.
- これらの発見は,単親のmtDNA遺伝の進化上の利点の潜在的な説明を提供します.
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