Jove
Visualize
お問い合わせ
JoVE
x logofacebook logolinkedin logoyoutube logo
JoVEについて
概要リーダーシップブログJoVEヘルプセンター
著者向け
出版プロセス編集委員会範囲と方針査読よくある質問投稿
図書館員向け
推薦の声購読アクセスリソース図書館諮問委員会よくある質問
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experimentsアーカイブ
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教員リソースセンター教員サイト
利用規約
プライバシーポリシー
ポリシー

関連する概念動画

siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...
Translational Regulation01:29

Translational Regulation

Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...
Non-LTR Retrotransposons03:18

Non-LTR Retrotransposons

As the name suggests, non-LTR retrotransposons lack the long terminal repeats characteristic of the LTR retrotransposons. Additionally, both LTR and non-LTR retrotransposons use distinct mechanisms of mobilization. Non-LTR retrotransposons are further divided into two classes - Long interspersed nuclear elements (LINEs) and short interspersed nuclear elements (SINEs), both of which occur abundantly in most mammals, including humans. Some of the active non-LTR retrotransposons in humans are L1...
lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA (lncRNA)...
lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA (lncRNA)...
Leaky Scanning02:28

Leaky Scanning

During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA.  Marilyn Kozak discovered that the sequence RCCAUGG (where R stands for...

こちらも読む

関連記事

共著者、ジャーナル、引用グラフによってこの研究に関連する記事。

並び替え
Same author

Vulvar Widening Through Perineal Flap Transposition, Rigottomy, And Fat Grafting For Vulvar Lichen Sclerosus.

Plastic and reconstructive surgery·2026
Same author

The myeloid SRC family kinase HCK regulates breast cancer growth by activating tumor-associated macrophage-led invasion and inhibiting cytotoxic T cell activity.

Frontiers in immunology·2026
Same author

Flt3L-mediated tumor cDC1 expansion enhances immunotherapy by priming stem-like CD8<sup>+</sup> T cells in lymph nodes.

Nature immunology·2026
Same author

Pharmacological targeting and characterization of Voltage-Gated Sodium Channels (VGSCs) expressed in the high-grade glioma microenvironment.

BMC cancer·2025
Same author

connectomeDB2025: a rigorously curated, multi-species resource of experimentally supported ligand-receptor interactions.

Nucleic acids research·2025
Same author

Impact of chemotherapy on humoral and cellular immune responses to COVID-19 vaccination in patients with solid tumors.

Frontiers in immunology·2025

関連する実験動画

Updated: May 17, 2026

Cell Based Assays of SINEUP Non-coding RNAs That Can Specifically Enhance mRNA Translation
10:21

Cell Based Assays of SINEUP Non-coding RNAs That Can Specifically Enhance mRNA Translation

Published on: February 1, 2019

長い非コーディングアンチセンセスのRNAは,埋め込まれたSINEB2の繰り返し経由でUchl1の翻訳を制御する.

Claudia Carrieri1, Laura Cimatti, Marta Biagioli

  • 1Area of Neuroscience, International School for Advanced Studies (SISSA), via Bonomea 265, 34136 Trieste, Italy.

Nature
|October 16, 2012
PubMed
まとめ

研究者らは,遺伝子発現を調節する新しいタイプの長いノンコーディングRNA (lncRNA) を発見した. このアンチセンセスのUchl1 lncRNAはタンパク質合成を強化し,転写後の遺伝子制御の新しい層を明らかにします.

さらに関連する動画

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA
09:36

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA

Published on: April 10, 2018

Enhanced Northern Blot Detection of Small RNA Species in Drosophila Melanogaster
09:39

Enhanced Northern Blot Detection of Small RNA Species in Drosophila Melanogaster

Published on: August 21, 2014

関連する実験動画

Last Updated: May 17, 2026

Cell Based Assays of SINEUP Non-coding RNAs That Can Specifically Enhance mRNA Translation
10:21

Cell Based Assays of SINEUP Non-coding RNAs That Can Specifically Enhance mRNA Translation

Published on: February 1, 2019

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA
09:36

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA

Published on: April 10, 2018

Enhanced Northern Blot Detection of Small RNA Species in Drosophila Melanogaster
09:39

Enhanced Northern Blot Detection of Small RNA Species in Drosophila Melanogaster

Published on: August 21, 2014

科学分野:

  • ゲノミクスゲノミクスとは
  • 分子生物学は分子生物学である.
  • 神経科学は神経科学である.

背景:

  • 哺乳類のゲノムの大半が転写され,長い非コーディングRNA (lncRNAs) を含む多様なRNA分子が生成されます.
  • 核濃縮のncRNAにはしばしば未知の機能がありますが,一部の反意味のlncRNAは,感覚と反意味のペアリングを通じて遺伝子発現を調節します.
  • ウビキチンカルボキシ末端ヒドロラーゼL1 (Uchl1) は,脳機能に不可欠であり,神経変性疾患に関与しています.

研究 の 目的:

  • 新型核濃縮 lncRNAsを特定し,特徴づけること.
  • Uchl1遺伝子に対する新たに特定されたlncRNAアンチセンスの機能を調査する.
  • Uchl1タンパク質合成の規制メカニズムを解明する.

主な方法:

  • トランスクリプトミア分析を用いた核濃縮のlncRNAの識別.
  • アンチセンセスのUchl1RNAとUchl1mRNAの相互作用の実験的検証.
  • mTORC1抑制を含む様々な条件下におけるUchl1タンパク質濃度の評価.
  • RNAの局所化とポリソーム関連性の分析.

主要な成果:

  • 新しい核濃縮のlncRNA,アンチセンセスのUchl1が特定されました.
  • Antisense Uchl1は,転写後のUchl1タンパク質合成を強化する.
  • その活動は,5'重複配列と埋め込まれたSINEB2要素に依存しています.
  • ラパミシン誘発のmTORC1阻害は,反感覚のUchl1を細胞質への輸出を促進し,翻訳を促進することによって,UCHL1タンパク質を増加させます.

結論:

  • 新しい機能性lncRNAsのクラス,アンチセンスUchl1が特定されました.
  • Antisense Uchl1は,転写後のレベルでのUchl1タンパク質合成を調節する.
  • ストレスシグナル伝達経路は,反感覚Uchl1の活性を調節し,UCHL1の翻訳に影響を与え,新しい遺伝子発現制御機構を明らかにします.