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LIN28Aは,胚性幹細胞におけるER関連翻訳の抑制剤である

Jun Cho¹, Hyeshik Chang¹, S Chul Kwon¹

¹Institute for Basic Science, Seoul National University, Seoul 151-742, Korea; School of Biological Sciences, Seoul National University, Seoul 151-742, Korea.

|October 30, 2012

まとめ

LIN28Aは,ERに定着したmRNAsの翻訳を阻害することによって,分泌経路内の遺伝子をグローバルに抑制します. このタンパク質,LIN28 (lin-28ホモログ) は,発達的移行やグルコース代謝にも影響する.

科学分野:

分子生物学は分子生物学である.
発達生物学発達生物学とは
がん研究がん研究

背景:

LIN28は,発達,代謝,がんに不可欠です.
LIN28はlet-7マイクロRNAの成熟を抑制し,特定のmRNAの翻訳を強化する.

研究の目的:

マウスの胚性幹細胞におけるLIN28Aの全ゲノム機能を解明する.
遺伝子調節とタンパク質合成におけるLIN28Aの役割を理解する.

主な方法:

RNAのクロスリンク・イムノプレシピテーション・シーケンシング (CLIP-seq).
リボソームの足跡.
LIN28A結合部位とターゲットmRNAの分析.

主要な成果:

LIN28Aは,let-7前駆体と多数のスプライスされたmRNAに結合する.
特定された LIN28A RNA 結合モチーフ: AAGNNG, AAGNG, UGUG.
LIN28Aは,エンドプラズマ網膜 (ER) の近くに位置し,ERを標的とするmRNAの翻訳を阻害する.
トランスメブラン,ER/ゴルギの光,および分泌タンパク質の合成が低下する.

結論:

LIN28Aは,分泌経路内の遺伝子のグローバル抑制剤として作用します.
ER関連翻訳のための選択的規制メカニズムを明らかにします.
let-7 規制を超えて,LIN28A の新たな役割を示唆しています.

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