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シリンドロサイクロファンの生物合成には,活性化されていない炭素中心の機能化が含まれています

Hitomi Nakamura¹, Hilary A Hamer, Gopal Sirasani

¹Department of Chemistry & Chemical Biology, Harvard University, Cambridge, Massachusetts 02138, United States.

Journal of the American Chemical Society

|October 31, 2012

まとめ

研究者らは,独特の炭素構造を持つ天然製品であるシリンドロサイクロファンFを生産する遺伝子のクラスタを特定した. この発見は,そのバイオシンセシスに関与する異常なポリケチド合成アセンブリとマクロサイクル形成に光を当てています.

科学分野:

自然製品バイオシンセシス自然製品バイオシンセシス
化学生物学化学生物学とは
ゲノミクスゲノミクスとは

背景:

シリンドロサイクロファンは,特徴的なパラサイクロファンの炭素基板によって特徴づけられる天然製品です.
複雑な天然製品の生合成を理解することは,薬剤発見と合成生物学にとって極めて重要です.

研究の目的:

シリンドロサイクロファンFの生産に責任のある生物合成遺伝子クラスタを特定する.
ポリケチド合成酵素活性とマクロサイクライゼーションを含む,シリンドロサイクロファンF組成の基礎となる酵素メカニズムを解明する.

主な方法:

ゲノムシーケンシングと生物情報分析により,遺伝子クラスタを特定する.
酵素の機能を決定するために,インビトロ酵素の特徴づけ.
遺伝子クラスターと産物形成の関連性を確認するための飼料研究.

主要な成果:

シリンドロサイクロファンのF生物合成に関与する特定の遺伝子クラスターが発見されました.
異常なタイプIポリケチド合成酵素の組み立てラインの開始と終了のステップが明らかにされました.
マクロサイクル形成は,活性化されていない炭素中心の機能化を伴うことが示されました.

結論:

特定された遺伝子クラスターは,円筒サイクロファンのF.生産に直接責任を負う.
この研究は,天然製品のバイオシンセシス,特にポリケチド組立とマクロサイクライゼーションで使用される新しい酵素戦略を明らかにしています.
この研究は,シリンドロサイクロファンの合成または半合成生産のための基礎を提供します.

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