スクラレールと珀の匂いを発する生物合成経路へ
Michel Schalk1, Laurence Pastore, Marco A Mirata
1Biotechnology Department, Corporate R&D Division, FIRMENICH SA, Geneva CH-1211, Switzerland. Michel.Schalk@firmenich.com
Journal of the American Chemical Society
|November 2, 2012
まとめ
研究者らは,E. coliを工学的に変えて,アンベルグライスの重要な代用品であるスクラレオルを発酵によって生成させた. この持続可能な方法は,香水成分のための伝統的な抽出に費用対効果の高い代替案を提供します.
科学分野:
- バイオテクノロジー バイオテクノロジー
- メタボリックエンジニアリング
- 合成生物学 合成生物学とは
背景:
- 価値ある香りの成分であるアンバーグリスは,供給不足と高コストに直面しています.
- アンブロックス (Ambrox) は,アンベルグリスの重要な代替品であり,クラレー・サージー (clary sage) から得られたスクラレオールから合成されます.
- スクラロールの持続可能で費用対効果の高い生産方法の開発は,香水業界にとって極めて重要です.
研究 の 目的:
- スクラレール生物合成に関与する酵素を特定し,特徴づけること.
- スクラレオルの生産のために微生物宿主を設計する.
- スクラロールの持続可能で費用対効果の高い発酵経路を確立する.
主な方法:
- スクラレール生物合成酵素のクローン化と機能的特徴化.
- Escherichia coli.におけるスクラレール生物合成経路の再構築
- スクラレール生産のために工学的に作られたE. coliの高細胞密度発酵.
主要な成果:
- スクラレール生物合成における重要な酵素を成功裏に特定し,特徴づけました.
- スクラレールを生成するE. coliをエンジニアリングし,タイターは約1.5g/Lに達します.
- スクラレール生産のための微生物発酵プロセスの実現可能性を実証しました.
結論:
- この研究は,スクラロールの持続可能で経済的な生産を開発するための基盤を提供します.
- スクラレールの微生物による生産は,植物抽出の有効な代替手段である.
- この研究は,バイオテクノロジーを用いて他の有価なテターペンの同類物を生産する道を開く.
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