メディエーターヘッドモジュールの構造
Laurent Larivière1, Clemens Plaschka, Martin Seizl
1Gene Center and Department of Biochemistry, Center for Integrated Protein Science Munich, Ludwig-Maximilians-Universität München, Feodor-Lynen-Straße 25, 81377 Munich, Germany. larivier@genzentrum.lmu.de
Nature
|November 6, 2012
まとめ
メディエーターのヘッドモジュールです.
科学分野:
- 分子生物学は分子生物学である.
- 構造生物学 構造生物学とは
- バイオケミストリー バイオケミストリー
背景:
- 媒介体複合体は,RNAポリメラーゼIIによる遺伝子転写に不可欠である.
- 転写レギュレータをRNAポリメラーゼIIと結びつけ,ヒトの疾患に関与している.
- 保存されたサブユニットで構成されるメディエーターヘッドモジュールは,この複合体の重要な構成要素です.
研究 の 目的:
- Schizosaccharomyces pombeのメディエーターヘッドモジュールの de novo結晶構造を決定するために.
- 分裂酵母メディエーターヘッドモジュールの詳細な建築モデルの提供.
- 媒介者の機能の構造的基礎と,遺伝子調節におけるその相互作用を解明する.
主な方法:
- 3.4 Å の解像度のX線結晶学.
- デノボ構造の決定.
- 既存のおよび新たに決定されたサブユニット構造の統合.
主要な成果:
- Schizosaccharomyces pombe Mediatorのヘッドモジュールの結晶構造を決定しました.
- ワニの頭のような構造を8つの異なる,部分的に移動可能な要素で明らかにしました.
- 固定した (歯と鼻) と首 (螺旋状の脊椎,肩,腕,指を伴う四肢) を含む主要な領域を特定しました.
- 構造は柔軟性と保全性を示唆し,RNAポリメラーゼIIとの相互作用に影響を及ぼします.
結論:
- 決定された構造は,Saccharomyces cerevisiae Mediatorのヘッドモジュールの改訂されたモデルを提供します.
- メディエーターヘッドモジュール内の高い保全と固有の柔軟性を強調します.
- 媒介者の機能に影響を与える突然変異についての洞察を提供し,遺伝子調節機構を理解するための枠組みを提供します.
関連する概念動画
Integrator and Differentiator
Op-amp circuits have significant applications in various fields, including automotive engineering. One such application is cruise control systems in cars, where op-amp circuits are integral for maintaining a constant speed. In these systems, op-amps function as both integrators and differentiators.
An integrator within an op-amp circuit produces an output directly proportional to the integral of the input signal. This is achieved by replacing the feedback resistor in a typical inverting...
An integrator within an op-amp circuit produces an output directly proportional to the integral of the input signal. This is achieved by replacing the feedback resistor in a typical inverting...
Mesh Analysis for AC Circuits
In the domain of radio communication, the significance of impedance matching must be considered. It is crucial to ensure the efficient transmission of signals between radio transmitters and receivers. Achieving this balance involves using impedance-matching circuits, with one fundamental configuration comprising a resistor, capacitor, and inductor.
The process of harmonizing these impedances begins with a clear understanding of the input and output signals. Once these signals are known, the...
The process of harmonizing these impedances begins with a clear understanding of the input and output signals. Once these signals are known, the...
Source Transformation for AC Circuits
The process of source transformation in the frequency domain entails the conversion of a voltage source, positioned in series with an impedance, into a current source that is parallel to an impedance, or the other way around. It is essential to maintain the following relationships while transitioning from one source type to another.
Bridge rectifier
The bridge rectifier is essential in electronics for efficiently converting alternating current (AC) to direct current (DC). Comprised of four diodes configured in a bridge layout, this rectifier effectively processes both the positive and negative halves of the AC waveform, making it superior to half-wave and full-wave center-tapped rectifiers in terms of voltage regulation and output stability.
Operationally, the bridge rectifier allows current flow through two of its diodes during each...
Operationally, the bridge rectifier allows current flow through two of its diodes during each...
Block Diagram Reduction
The process of deriving the transfer function of a control system often involves reducing its block diagram to a single block. This simplification can be achieved through a series of strategic operations, including relocating branch points and comparators. These operations preserve the overall function of the system while allowing for easier manipulation and combination of blocks.
The first step in this process is the identification and relocation of a branch point. A branch point, where a...
The first step in this process is the identification and relocation of a branch point. A branch point, where a...
Bus Impedance Matrix
Calculating subtransient fault currents for three-phase faults in an N-bus power system involves using the positive-sequence network. When a three-phase short circuit occurs at a specific bus, the analysis uses the superposition method to evaluate two separate circuits.
In the first circuit, all machine voltage sources are short-circuited, leaving only the prefault voltage source at the fault location. The positive-sequence bus impedance matrix can be determined by solving the nodal equations,...
In the first circuit, all machine voltage sources are short-circuited, leaving only the prefault voltage source at the fault location. The positive-sequence bus impedance matrix can be determined by solving the nodal equations,...


