変動するフィールド条件下でのトランスクリプトーム動態の解読と予測
Atsushi J Nagano1, Yutaka Sato, Motohiro Mihara
1Photosynthesis and Photobiology Research Unit, National Institute of Agrobiological Sciences, 2-1-2 Kannondai, Tsukuba, Ibaraki 305-8602, Japan.
Cell
|December 11, 2012
まとめ
米の植物は,米の植物は,米の植物は,米の植物は.
科学分野:
- 植物生物学 植物生物学
- ゲノミクスゲノミクスとは
- 環境科学 環境科学
背景:
- 遺伝子発現は,実験室と比較して自然環境では複雑です.
- 植物遺伝子発現に対する環境の影響を理解することは,農業にとって極めて重要です.
研究 の 目的:
- 米畑での米植物の遺伝子発現に影響を与える内在的および外部要因をモデリングする.
- 自然環境におけるトランスクリプトームのダイナミクスの主要な要因を特定する.
主な方法:
- 米葉からトランスクリプトームデータを収集した.
- 関連する気象データを収集した.
- 遺伝子発現の要因を分析するための統計モデルを開発.
主要な成果:
- トランスクリプトームの動態は,主に昼間のリズム,温度,植物の年齢,太陽放射線によって左右されます.
- 環境要因が遺伝子転写に影響を与える特定の時間 (昼間ゲート) を特定します.
- 代謝遺伝子に対する昼夜と環境要因の相対的な影響を定量化した.
結論:
- 米のトランスクリプトームの動態に対する温度効果の予測モデルを開発した.
- この研究は,農業の課題に適用可能な洞察を提供します.
- このアプローチは,他の生物におけるトランスクリプトームの変動を解読するために拡張することができます.
関連する概念動画
01:10Transcription
Overview
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
01:29Translational Regulation
Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...
02:51mRNA Stability and Gene Expression
The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
Cis-acting Elements involved in mRNA stability
02:51mRNA Stability and Gene Expression
The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
Cis-acting Elements involved in mRNA stability
02:24Ribosome Profiling
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...