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関連する概念動画

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
CRISPR01:59

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...
CRISPR01:59

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...
CRISPR and crRNAs02:53

CRISPR and crRNAs

Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...

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関連する実験動画

Updated: May 15, 2026

CRISPR/Cas12a Multiplex Genome Editing of Saccharomyces cerevisiae and the Creation of Yeast Pixel Art
10:18

CRISPR/Cas12a Multiplex Genome Editing of Saccharomyces cerevisiae and the Creation of Yeast Pixel Art

Published on: May 28, 2019

CRISPR/Casシステムを用いたマルチプレックスゲノム工学.

Le Cong1, F Ann Ran, David Cox

  • 1Broad Institute of MIT and Harvard, 7 Cambridge Center, Cambridge, MA 02142, USA.

Science (New York, N.Y.)
|January 5, 2013
PubMed
まとめ
この要約は機械生成です。

この研究は,ヒトとマウスの細胞における正確なDNA分裂のためのCRISPR-Cas9遺伝子編集を導入しています. この技術により,複数のゲノムサイトを同時に編集でき,遺伝子研究に広く適用できます.

さらに関連する動画

Genome Editing in Mammalian Cell Lines using CRISPR-Cas
07:56

Genome Editing in Mammalian Cell Lines using CRISPR-Cas

Published on: April 11, 2019

A Protocol for Multiple Gene Knockout in Mouse Small Intestinal Organoids Using a CRISPR-concatemer
11:53

A Protocol for Multiple Gene Knockout in Mouse Small Intestinal Organoids Using a CRISPR-concatemer

Published on: July 12, 2017

関連する実験動画

Last Updated: May 15, 2026

CRISPR/Cas12a Multiplex Genome Editing of Saccharomyces cerevisiae and the Creation of Yeast Pixel Art
10:18

CRISPR/Cas12a Multiplex Genome Editing of Saccharomyces cerevisiae and the Creation of Yeast Pixel Art

Published on: May 28, 2019

Genome Editing in Mammalian Cell Lines using CRISPR-Cas
07:56

Genome Editing in Mammalian Cell Lines using CRISPR-Cas

Published on: April 11, 2019

A Protocol for Multiple Gene Knockout in Mouse Small Intestinal Organoids Using a CRISPR-concatemer
11:53

A Protocol for Multiple Gene Knockout in Mouse Small Intestinal Organoids Using a CRISPR-concatemer

Published on: July 12, 2017

科学分野:

  • 分子生物学は分子生物学である.
  • 遺伝学 遺伝学とは
  • バイオテクノロジー バイオテクノロジー

背景:

  • 精密なゲノム編集は,遺伝的変異を理解するために不可欠です.
  • プロカリオットのCRISPR/Casシステムは,RNA主導のDNA分裂能力を提供しています.

研究 の 目的:

  • 哺乳類の細胞における標的ゲノム編集のためのCRISPR-Cas9システムの有効性を設計し,実証する.
  • 機能的ゲノミクスのためのRNA誘導核酸のプログラム性と適用性を探求する.

主な方法:

  • 2つの異なるタイプIIのCRISPR/Casシステムのエンジニアリング.
  • サイト固有のDNA分裂のために,短いRNAによって導かれるCas9核素を用いる.
  • Cas9をニッキング酵素に変換して,同位性指向の修復を行う.

主要な成果:

  • エンジニアリングされたCRISPR-Cas9システムを使用して,ヒトとマウスの細胞の内生的なゲノムロシに正確な割れ目を実証しました.
  • 改善された修復のためのニッキングCas9変異体との最小限の変異性活性を示した.
  • 単一のCRISPR配列を介して同時に複数のサイトでゲノム編集を可能にし,プログラミング性を強調しました.

結論:

  • エンジニアリングされたRNA誘導型CRISPR-Cas9核酵素技術は高度にプログラム可能であり,哺乳類のゲノム編集に広く適用できます.
  • このシステムは,遺伝子変異や要素の機能的解明を,精度と効率で容易にします.