肝臓で濃縮された転写活性化剤であるDBPは,本体生成の遅い段階で発現し,その組織特異性は,転写後の方法で決定されます

C R Mueller¹, P Maire, U Schibler

¹Department of Molecular Biology, University of Geneva, Switzerland.

Cell

|April 20, 1990

まとめ

アルブミン遺伝子調節に関与するタンパク質である転写活性化剤DBPは,成人の肝臓において高い発現率を有する. 肝臓再生中にその発現は低下調節され,肝細胞増殖制御における役割を示唆しています.

科学分野:

分子生物学は分子生物学である.
遺伝子規制遺伝子規制
肝臓細胞の機能について

背景:

アルブミンプロモーターには,転写活性化剤によって認識されるDサイトが含まれています.
転写因子は遺伝子発現の重要な調節因子である.
DBPタンパク質ファミリーは基本的なドメインを共有していますが,DBPにはルシンジッパーがありません.

研究の目的:

転写活性化剤のDBPをクローンし,特徴づけること.
DBPの組織分布と発達の表現を調査する.
肝臓の再生と細胞増殖におけるDBPの役割を調査する.

主な方法:

DBPの全長cDNAクローニング.
様々なネズミの組織におけるDBPタンパク質とmRNA濃度の分析.
化学的に誘発された肝臓再生中のDBP発現の検査.

主要な成果:

DBPのcDNAがクローンされ,Fos,Jun,CREB,C/EBPに関連する転写因子を明らかにした.
DBPタンパク質は主に成人のラット肝臓に存在し,そのmRNAは広く分布しているが,成人に蓄積する.
DBP発現は,肝臓の再生中に急速に下調され,この調節はmRNAレベルで行われます.

結論:

DBPは,アルバミンプロモーターのD部位に結合する新しい転写活性化剤です.
DBPは,主に成人の肝臓において,組織特異的および発達的調節を示す.
肝臓再生中のDBPのダウンレギュレーションは,肝細胞の増殖を制御する役割を示唆しています.

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