構造的にはTFIIIAに似た指タンパク質で,Xenopususの5SRNAにのみ結合する

K E Joho¹, M K Darby, E T Crawford

¹Department of Embryology, Carnegie Institution of Washington, Baltimore, Maryland 21210.

Cell

|April 20, 1990

まとめ

Xenopus卵細胞には5SRNA結合タンパク質 (p43) が含まれており,TFIIIAと構造的に類似しています. TFIIIAとは異なり,p43は5SRNAのみを結合し,遺伝子を結合しません.

科学分野:

分子生物学は分子生物学である.
発達生物学発達生物学について
Xenopus laevisに関する研究が行われている.

背景:

5SRNA結合タンパク質であるp43は,Xenopusの卵細胞に豊富に含まれています.
p43は42Sリボヌクレオプロテインの貯蔵粒子の構成要素である.

研究の目的:

Xenopus laevisとXenopus borealisからp43cDNAをクローンして配列化する.
p43を別の5SRNA結合タンパク質であるTFIIIAと比較するには.

主な方法:

Xenopusからp43とTFIIIAのcDNAクローニングとシーケンシング.
アミノ酸配列解析とホモロジーの比較.

主要な成果:

p43とTFIIIAは,9つの亜鉛指を含む構造的な類似性を共有しています.
p43の7本の亜鉛指は,TFIIIAの7本の亜鉛指と同じサイズです.
アミノ酸ホモロジーは,保存された亜鉛指の残留物に濃縮されています.
p43は5SRNAにのみ結合するが,TFIIIAは5SRNAとその遺伝子に結合する.

結論:

p43は,特定のRNA結合機能を持つ独特の5SRNA結合タンパク質です.
構造的な類似性は,p43とTFIIIAの間の共通の進化的起源または機能的な関係を示唆しています.

さらに関連する動画

09:38

Identification of Nucleolar Factors During HIV-1 Replication Through Rev Immunoprecipitation and Mass Spectrometry

Published on: June 26, 2019

11:34

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins

Published on: August 9, 2019

関連する概念動画

01:23

RNA Structure

Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...

02:36

RNA Polymerase II Accessory Proteins

Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...

01:56

Riboswitches

Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...

01:19

RNA Structure

The basic structure of RNA consists of a string of ribonucleotides attached by phosphodiester bonds. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...

01:30

General Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...