ヒストンmRNA幹ループ,ヒト幹ループ結合タンパク質,および3'hExo三元複合体の構造
Dazhi Tan1, William F Marzluff, Zbigniew Dominski
1Department of Biological Sciences, Columbia University, New York, NY 10027, USA.
Science (New York, N.Y.)
|January 19, 2013
まとめ
結晶構造は,幹回路結合タンパク質 (SLBP) と3の結合方法を明らかにしています.
科学分野:
- 分子生物学は分子生物学である.
- 構造生物学 構造生物学とは
- バイオケミストリー バイオケミストリー
背景:
- 複製に依存するヒストンのmRNAは,3'-end stem-loop (SL) 構造を保持しています.
- 幹ループ結合タンパク質 (SLBP) は,SL.を結合することによってヒストンのmRNA代謝を調節する.
- 3'-5'エクソヌクレアゼ (3'hExo) はヒストンmRNAの3'-エンドをトリムする.後処理.
研究 の 目的:
- SLBP,3'hExoとSLRNAの相互作用の構造的基礎を決定する.
- ヒストン mRNA 3'-end 処理と調節のメカニズムを解明する.
主な方法:
- 三元複合体のX線結晶学.
- タンパク質-RNAの相互作用と酵素活性を評価するための生化学的測定法.
主要な成果:
- 結晶構造は,ヒトSLBPRNA結合ドメイン,ヒト3'hExo,および26核酸SLRNAの三元複合体を明らかにしています.
- SLBPは,SLRNAの単一の塩基を特異的に認識しますが,両方のタンパク質は主にRNAの全体的な形状を認識します.
- SLBPと3'hExoは互いに直接接触することはなく,SLRNAループに誘発された構造的変化によって協同結合が媒介される.
- 3'側面配列は3'hExo活性部位に配置されていますが,複雑な形成により,トリミングが制限されます.
結論:
- この構造は,SLBPと3'hExo.によるヒストンのmRNA処理の調整された調節に関する洞察を提供します.
- この発見は,タンパク質の認識と複合体の形成におけるRNAの形状の互補性の重要性を強調しています.
- 三重複合体の構造は,3'hExoの活性がヒストンのmRNA代謝中にどのように調節されるかを説明する.
関連する概念動画
01:19RNA Structure
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Different Types of RNA Have the Same Basic Structure
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Different Types of RNA Have the Same Basic Structure
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01:23RNA Structure
Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
01:23RNA Structure
Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
01:12The Nucleosome Core Particle
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Nucleosomes, paradoxically, perform two opposite functions simultaneously. On the one hand, their primary aim is to protect the delicate DNA strands from physical damage and help achieve a higher compaction ratio. On the other hand, they must allow polymerase enzymes to access histone-bound DNA during...
02:10The Nucleosome Core Particle
Nucleosomes are the DNA-histone complex, where the DNA strand is wound around the histone core. The histone core is an octamer containing two copies of H2A, H2B, H3, and H4 histone proteins.
The paradox
Nucleosomes, paradoxically, perform two opposite functions simultaneously. On the one hand, their main responsibility is to protect the delicate DNA strands from physical damage and help achieve a higher compaction ratio. While on the other hand, they must allow polymerase enzymes to access DNA...
The paradox
Nucleosomes, paradoxically, perform two opposite functions simultaneously. On the one hand, their main responsibility is to protect the delicate DNA strands from physical damage and help achieve a higher compaction ratio. While on the other hand, they must allow polymerase enzymes to access DNA...
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Acetylation
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