人間のTHAP9遺伝子は,活性P要素DNAトランポザゼをコードする

Sharmistha Majumdar¹, Anita Singh, Donald C Rio

¹Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720-3204, USA.

Science (New York, N.Y.)

|January 26, 2013

まとめ

人間のTHAP9は,家畜の遺伝子であり,活性DNAトランスポゼです. ヒトとハエの細胞の両方でドロソフィラP元素を動員することができ,種間活動を示しています.

科学分野:

分子生物学は分子生物学である.
遺伝学遺伝学とは
進化生物学の進化生物学について

背景:

人間のゲノムには,移植可能な元素 (TEs) から発生した約50の遺伝子が含まれています.
これらの家畜化されたTEsの多くは,現在,細胞の遺伝子発現に不可欠です.
人間のTHAP9遺伝子は,ドロソフィラのP要素トランポゼスと同質性を共有しています.

研究の目的:

人間のTHAP9遺伝子の機能的活動を調査する.
人間のTHAP9が転置可能な要素を動員できるかどうかを判断する.
ドロソフィラP元素に関連したTHAP9の種間活動を探求する.

主な方法:

アッセイは,ドロソフィラとヒトの両方の細胞系で行われました.
人間のTHAP9がドロソフィラP元素を動員する能力がテストされました.
THAP9ドメインとP要素トランポザーゼ領域を組み合わせたキメリックタンパク質が設計され,テストされました.

主要な成果:

人間THAP9は,ドロソフィラとヒトの細胞の両方でドロソフィラP元素を動員する能力を実証しました.
THAPのDNA結合ドメインとTHAP9のC末端領域を含むキメリックタンパク質もP元素を動員した.
これらの発見は,THAP9が,種間の動員能力を持つ活性DNAトランポザースであることを確認しています.

結論:

人間のTHAP9は,活性DNAトランスポゼとして機能し,家畜化にもかかわらず,触媒的活動を保持しています.
THAP9は,ドロソフィラP元素の異なった種間の転置を媒介することができる.
この研究は,真核生物における古代のトランポゾン由来遺伝子の機能能力が保持されていることを強調しています.

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