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関連する概念動画

Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Lineage Commitment01:21

Lineage Commitment

Commitment is the  process whereby stem cells:
lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA (lncRNA)...
lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA (lncRNA)...
RACE - Rapid Amplification of cDNA Ends02:35

RACE - Rapid Amplification of cDNA Ends

Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific primer.
Since the...

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関連する実験動画

Updated: May 14, 2026

High Efficiency Differentiation of Human Pluripotent Stem Cells to Cardiomyocytes and Characterization by Flow Cytometry
13:13

High Efficiency Differentiation of Human Pluripotent Stem Cells to Cardiomyocytes and Characterization by Flow Cytometry

Published on: September 23, 2014

Braveheartは,心臓血管系統のコミットメントに必要な長いノンコーディングRNAです.

Carla A Klattenhoff1, Johanna C Scheuermann, Lauren E Surface

  • 1Department of Biology, Massachusetts Institute of Technology, 77 Massachusetts Avenue, Cambridge, MA 02139, USA.

Cell
|January 29, 2013
PubMed
まとめ

ブレイブハート (Bvht) は,新しい長いノンコーディングRNAであり,心臓血管系統の確立に不可欠です. この心臓に関連したlncRNAは,胚の幹細胞の分化を心臓の運命を導いて,重要な発達遺伝子を調節します.

関連する実験動画

Last Updated: May 14, 2026

High Efficiency Differentiation of Human Pluripotent Stem Cells to Cardiomyocytes and Characterization by Flow Cytometry
13:13

High Efficiency Differentiation of Human Pluripotent Stem Cells to Cardiomyocytes and Characterization by Flow Cytometry

Published on: September 23, 2014

科学分野:

  • 発達生物学 発達生物学について
  • エピジェネティクス エピジェネティクス
  • RNA 生物学 RNA 生物学

背景:

  • 長い非コーディングRNA (lncRNAs) は発達において役割を果たしますが,系統のコミットメントにおけるその機能はほとんど不明です.
  • 心血管発達の分子メカニズムを理解することは,再生医療にとって極めて重要です.

研究 の 目的:

  • 心血管系系への関与に関与する新しいlncRNAを特定し,特徴づけること.
  • 哺乳類の心臓発育における心臓に関連したlncRNA Braveheart (Bvht) の機能を明らかにする.

主な方法:

  • 胚性幹細胞 (ESC) 微分化アッセイ. 胚性幹細胞 (ESC) 微分化アッセイ.
  • 遺伝子発現分析 (qRT-PCR,RNA-seq) について
  • クロマチン免疫プレシピテーション (ChIP) アッセイは,表遺伝的変化を評価するためのものです.
  • SUZ12とMesP1.1との相互作用に関する研究

主要な成果:

  • Bvhtは,心筋系にメソダーマの分化に不可欠です.
  • Bvhtは,心臓血管系の中核の遺伝子ネットワークを調節し,MesP1.1の上流に作用する.
  • Bvhtは,PRC2の成分であるSUZ12と相互作用し,表遺伝子調節における役割を示唆しています.
  • Bvhtは,新生児の心筋細胞における心臓の運命を維持することにも関わっています.

結論:

  • Braveheart (Bvht) は,哺乳類の発達中に心血管系を確立するための重要な長いノンコーディングRNAである.
  • Bvhtは,PRC2を含む表遺伝的メカニズムを通じて,心臓のコミットメントを調節するために機能します.
  • この研究は,発育過程におけるlncRNAsの重要性を強調し,心臓研究のための新しいターゲットを提供します.