信号活性化ストキャスティック遺伝子調節の体系的な識別
Gregor Neuert1, Brian Munsky, Rui Zhen Tan
1Departments of Physics and Biology and Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
まとめ
この研究は,酵母における遺伝子転写ダイナミクスの予測モデルを開発しています. このアプローチは,単細胞実験とストキャスティック分析を統合して,オスモティックストレスに対する細胞反応を理解します.
科学分野:
- システム生物学 システム生物学
- 分子生物学は分子生物学である.
- コンピュータ生物学 コンピュータ生物学
背景:
- 信号伝達と遺伝子調節経路における定量的な反応を理解することは,依然として困難です.
- 分子機構に基づいて細胞の行動を予測するには,洗練されたモデリングアプローチが必要です.
研究 の 目的:
- オスモティックストレス反応経路における転写ダイナミクスに関する予測モデルの開発と検証.
- ストレス反応中の遺伝子発現を制御する規制メカニズムについての洞察を得るために.
- 信号活性化トランスクリプションを予測するための一般化可能な枠組みを確立する.
主な方法:
- 単細胞実験データの統合とストキャスティック分析.
- 複雑性が異なるモデルの開発と比較.
- モデル選択のためのパラメータ推定とクロス検証.
主要な成果:
- Saccharomyces cerevisiaeにおける転写ダイナミクスの予測モデルの特定.
- 多段階調節やスイッチ状の活性化などのオスモセンシティブ遺伝子のダイナミックな特徴を明らかにした.
- このモデルは,環境的および遺伝的混乱に対する転写反応を正確に予測した.
結論:
- 開発されたアプローチは,信号活性化トランスクリプションの予測的理解を容易にする.
- この方法論は,他の遺伝子,経路,生物にも適用できます.
- 遺伝子調節ダイナミクスを理解する上でストキャスティシティの重要性を強調する.
関連する概念動画
Global Regulatory Systems
Global regulatory systems in bacteria enable rapid and coordinated responses to environmental changes by integrating sensory inputs with gene expression, ensuring efficient adaptation to fluctuating conditions. Key global regulatory mechanisms include regulons, two-component systems, sigma factors, and secondary messengers.Regulons and Global RegulatorsA regulon is a collection of genes and operons controlled by a common global regulator. These regulators enable bacteria to prioritize resource...
Reporter Genes
Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...
Commonly used reporter...
Gene Regulation During Sporulation
Sporulation is a complex developmental process that allows certain Gram-positive bacteria, such as Bacillus subtilis and Clostridium species, to survive extreme environmental conditions. This process is tightly regulated by a series of signaling cascades and transcriptional controls, ensuring the formation of a highly resistant endospore.Sporulation is triggered by unfavorable conditions, such as nutrient depletion, and is governed by a phosphorelay system. One of the sensor kinases, such as...
Combinatorial Gene Control
Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
Regulation of Expression at Multiple Steps
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the addition of a...
Regulation of Expression Occurs at Multiple Steps
Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...


