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関連する概念動画

Allosteric Proteins-ATCase01:19

Allosteric Proteins-ATCase

Binding sites linkages can regulate a protein's function.  For example, enzyme activity is often regulated through a feedback mechanism where the end product of the biochemical process serves as an inhibitor.
Aspartate transcarbamoylase (ATCase) is a cytosolic enzyme that catalyzes the condensation of L-aspartate and carbamoyl phosphate to  N-carbamoyl-L-aspartate. This reaction is the first step in pyrimidine biosynthesis. UTP and CTP, the end products of the pyrimidine synthesis pathway,...
Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...
Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
Allosteric Regulation01:08

Allosteric Regulation

Allosteric regulation of enzymes occurs when the binding of an effector molecule to a site that is different from the active site causes a change in the enzymatic activity. This alternate site is called an allosteric site, and an enzyme can contain more than one of these sites. Allosteric regulation can either be positive or negative, resulting in an increase or decrease in enzyme activity. Most enzymes that display allosteric regulation are metabolic enzymes involved in the degradation or...
Allosteric Regulation01:08

Allosteric Regulation

Allosteric regulation of enzymes occurs when the binding of an effector molecule to a site that is different from the active site causes a change in the enzymatic activity. This alternate site is called an allosteric site, and an enzyme can contain more than one of these sites. Allosteric regulation can either be positive or negative, resulting in an increase or decrease in enzyme activity. Most enzymes that display allosteric regulation are metabolic enzymes involved in the degradation or...
From DNA to Protein03:06

From DNA to Protein

The flow of genetic information in cells from DNA to mRNA to protein is described by the central dogma, which states that genes specify the sequence of mRNAs, which in turn specify the sequence of amino acids making up all proteins. The decoding of one molecule to another is performed by specific proteins and RNAs. Because the information stored in DNA is so central to cellular function, it makes intuitive sense that the cell would make mRNA copies of this information for protein synthesis...

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Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells
14:02

Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells

Published on: April 9, 2018

DNAを通してアロステリーを検知する.

Sangjin Kim1, Erik Broströmer, Dong Xing

  • 1Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA.

Science (New York, N.Y.)
|February 16, 2013
PubMed
まとめ
この要約は機械生成です。

研究者は,近くのタンパク質がDNAとタンパク質の結合を変化させるDNAアロステリーを発見しました. この相互作用は,相互作用です.

さらに関連する動画

An Allele-specific Gene Expression Assay to Test the Functional Basis of Genetic Associations
10:17

An Allele-specific Gene Expression Assay to Test the Functional Basis of Genetic Associations

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Residue-Specific Exchange of Proline by Proline Analogs in Fluorescent Proteins: How "Molecular Surgery" of the Backbone Affects Folding and Stability
10:31

Residue-Specific Exchange of Proline by Proline Analogs in Fluorescent Proteins: How "Molecular Surgery" of the Backbone Affects Folding and Stability

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関連する実験動画

Last Updated: May 14, 2026

Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells
14:02

Optimizing the Genetic Incorporation of Chemical Probes into GPCRs for Photo-crosslinking Mapping and Bioorthogonal Chemistry in Live Mammalian Cells

Published on: April 9, 2018

An Allele-specific Gene Expression Assay to Test the Functional Basis of Genetic Associations
10:17

An Allele-specific Gene Expression Assay to Test the Functional Basis of Genetic Associations

Published on: November 3, 2010

Residue-Specific Exchange of Proline by Proline Analogs in Fluorescent Proteins: How "Molecular Surgery" of the Backbone Affects Folding and Stability
10:31

Residue-Specific Exchange of Proline by Proline Analogs in Fluorescent Proteins: How "Molecular Surgery" of the Backbone Affects Folding and Stability

Published on: February 3, 2022

科学分野:

  • 分子生物学は分子生物学である.
  • バイオフィジックス 生物物理学
  • 遺伝学 遺伝学とは

背景:

  • 遠隔結合イベントによるタンパク質機能の調節であるアロステリーは,タンパク質にとってよく確立されています.
  • DNAとタンパク質の相互作用におけるアロステリック効果は,理解が薄いままであり,重要な知識のギャップを表しています.
  • DNAとタンパク質の相互作用を理解することは,遺伝子調節と細胞プロセスにとって極めて重要です.

研究 の 目的:

  • DNAとタンパク質の相互作用におけるアロステリー現象を調査し,特徴づけること.
  • DNAアロステリック効果の物理的根拠と周期性を決定する.
  • 生物系におけるDNAアロステリーの生理学的影響を調査する.

主な方法:

  • 異なるタンパク質-タンパク質分離下でタンパク質-DNA結合親和性の実験的決定.
  • タンパク質間の距離の関数として三元複合体の自由エネルギーの分析.
  • DNAアロステリーの構造的基礎を解明するための分子動力学シミュレーション.
  • 遺伝子発現への影響を評価するために,細菌におけるインビボ研究.

主要な成果:

  • タンパク質とDNAの特定の結合は,近くのタンパク質の存在によって著しく安定化または不安定化されます.
  • 三元複合体の自由エネルギーは,約10塩基対の周期性 (B-DNAの螺旋ピッチ) で振動する.
  • DNAアロステリーは,シミュレーションによって裏付けられているように,DNAの二重ヘリル構造の変形から発生します.
  • この現象はバクテリアの遺伝子発現に影響を与え,ヌクレオソーム近くの転写因子結合に影響を与えます.

結論:

  • DNAアロステリーは,DNAとタンパク質の相互作用を調節する新しく認識されたメカニズムです.
  • DNAの螺旋構造は,これらのアロステリック効果を媒介する上で重要な役割を果たします.
  • DNAアロステリーは,遺伝子発現とタンパク質結合の調節において,生体内で重要な生理学的関連性を持っています.