NeSTの長いncRNAは,微生物の感受性と,インターフェロン-γのロカスにおける表遺伝的活性化を制御する
J Antonio Gomez1, Orly L Wapinski, Yul W Yang
1Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305, USA.
Cell
|February 19, 2013
まとめ
新しい長いノンコーディングRNAであるNeSTは,遺伝子発現と免疫反応を調節する. NeSTは,インターフェロン-ガンマロキュスの表遺伝子マーキングを変更することによって,ウイルスおよび細菌の病原体に対する感受性を制御します.
科学分野:
- 免疫学 免疫学とは
- 遺伝学 遺伝学とは
- 分子生物学は分子生物学である.
背景:
- 長いノンコーディングRNA (lncRNAs) は,遺伝子発現の重要な調節因子として認識されています.
- ネズミのウイルス感受性位置Tmevp3は,宿主の病原体に対する反応に影響を与えます.
- SJL/Jマウスは,B10.Sマウスと比較して,独特の免疫フェノタイプを示しています.
研究 の 目的:
- Tmevp3ロカス内の因果遺伝子を特定するために.
- ホスト防御における特定されたlncRNAの機能を明らかにする.
- NeST媒介による遺伝子調節の分子機構を理解する.
主な方法:
- SJL/JとB10.Sのマウスの間の遺伝子クロスで,Tmevp3ロクスを定義する.
- NeST lncRNA.のトランスジェニック発現
- タンパク質相互作用体を特定するためのRNA結合測定法.
- ヒストンの改変を評価するためのクロマチン免疫プレシピテーション.
主要な成果:
- 強化剤のようなlncRNA NeSTは,Tmevp3フェノタイプの原因因子として特定されました.
- トランスジェニックのNeST発現だけで,SJL/Jロカス関連フェノタイプをすべて再現した.
- NeST RNAはWDR5と結合し,インターフェロン-ガンマ (IFN-γ) 場所でのヒストンのメチル化を変化させます.
- NeSTは,IFN-γの発現と,Theiler's virusとSalmonella entericaに対する感受性を調節する.
結論:
- NeSTは,免疫反応と病原体の感受性の重要なレギュラーです.
- このlncRNAは,IFN-γ発現を制御するために表遺伝的に作用します.
- NeSTは,感染症に対する新しい治療目標です.
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