遺伝子的に定義された2つのトランス作用局は,肝臓特異的な遺伝子の重複するセットを協調的に調節します
S Ruppert1, M Boshart, F X Bosch
1Institute of Cell and Tumor Biology, German Cancer Research Center, Heidelberg.
Cell
|June 1, 1990
まとめ
マウスのアルフトランス作用因子の喪失は,新生児の肝機能を乱し,致死性を引き起こします. グルココルチコイドとcAMPは,この効果を逆転させ,alfとTse-1による調整された遺伝子調節を明らかにすることができます.
科学分野:
- 分子生物学は分子生物学である.
- 発達生物学 発達生物学について
- 遺伝学 遺伝学とは
背景:
- アルビノ位置の近くで欠損したマウスは,致命的な新生児現象型を示しています.
- このフェノタイプは,トランス作用因子,alf.の喪失と関連しています.
- 新生児の肝機能は,アルファ欠乏症のマウスで低下しています.
研究 の 目的:
- ALFトランス作用因子によって調節される遺伝子を特定する.
- アルファ媒介遺伝子発現におけるグルココルチコイドとcAMPの役割を調査する.
- alfと組織特異的な消火器の場所 Tse-1 の間の相互作用を探求する.
主な方法:
- アルフ反応性遺伝子を隔離するために差分cDNAスクリーニング.
- ホルモン刺激に反応する遺伝子発現パターンの分析.
- アルフ調節遺伝子に対するTse-1の効果を調査した.
主要な成果:
- 転写がalf.によって影響を受ける遺伝子を特定した.
- アルフ反応性遺伝子は,グルココルチコイドとcAMPによって誘発される.
- Tse-1がアルファ反応性遺伝子のサブセットを否定的に制御することを発見した.
- グルココルチコイドとcAMPがTse-1-媒介抑制を克服できることを実証しました.
結論:
- 2つのトランス作用因子,alfとTse-1は,肝臓特異的な遺伝子セットの重複の調節を調整する.
- 致死性フェノタイプと消滅した遺伝子発現は,ホルモン信号伝達経路の障害による可能性が高い.
さらに関連する動画
11:35Screening for Functional Non-coding Genetic Variants Using Electrophoretic Mobility Shift Assay (EMSA) and DNA-affinity Precipitation Assay (DAPA)
Published on: August 21, 2016
09:35Constitutive and Inducible Systems for Genetic In Vivo Modification of Mouse Hepatocytes Using Hydrodynamic Tail Vein Injection
Published on: February 2, 2018
関連する概念動画
02:16Transcription Factors
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
02:08Gene Conversion
Other than maintaining genome stability via DNA repair, homologous recombination plays an important role in diversifying the genome. In fact, the recombination of sequences forms the molecular basis of genomic evolution. Random and non-random permutations of genomic sequences create a library of new amalgamated sequences. These newly formed genomes can determine the fitness and survival of cells. In bacteria, homologous and non-homologous types of recombination lead to the evolution of new...
01:58Cell Specific Gene Expression
Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
02:33Combinatorial Gene Control
Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
01:30General Transcription Factors
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
02:08Gene Conversion
Other than maintaining genome stability via DNA repair, homologous recombination plays an important role in diversifying the genome. In fact, the recombination of sequences forms the molecular basis of genomic evolution. Random and non-random permutations of genomic sequences create a library of new amalgamated sequences. These newly formed genomes can determine the fitness and survival of cells. In bacteria, homologous and non-homologous types of recombination lead to the evolution of new...