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関連する概念動画

RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
Nucleic Acids02:43

Nucleic Acids

Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes, the...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
Ribozymes02:47

Ribozymes

The term ribozyme is used for RNA that can act as an enzyme. Ribozymes are mainly found in selected viruses, bacteria, plant organelles, and lower eukaryotes. Ribozymes were first discovered in 1982 when Tom Cech’s laboratory observed Group I introns acting as enzymes. This was shortly followed by the discovery of another ribozyme, Ribonulcease P, by Sid Altman’s laboratory. Both Cech and Altman received the Nobel Prize in chemistry in 1989 for their work on ribozymes.
Ribozymes can be...
Ribozymes02:47

Ribozymes

The term ribozyme is used for RNA that can act as an enzyme. Ribozymes are mainly found in selected viruses, bacteria, plant organelles, and lower eukaryotes. Ribozymes were first discovered in 1982 when Tom Cech’s laboratory observed Group I introns acting as enzymes. This was shortly followed by the discovery of another ribozyme, Ribonulcease P, by Sid Altman’s laboratory. Both Cech and Altman received the Nobel Prize in chemistry in 1989 for their work on ribozymes.
Ribozymes can be...
Nucleic Acid Structure01:25

Nucleic Acid Structure

The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms  a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA has a double-helix structure. The...

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関連する実験動画

Updated: May 13, 2026

Highly Efficient Ligation of Small RNA Molecules for MicroRNA Quantitation by High-Throughput Sequencing
14:15

Highly Efficient Ligation of Small RNA Molecules for MicroRNA Quantitation by High-Throughput Sequencing

Published on: November 18, 2014

自然のRNAサークルは,効率的なマイクロRNAスポンジとして機能します.

Thomas B Hansen1, Trine I Jensen, Bettina H Clausen

  • 1Department of Molecular Biology and Genetics, Aarhus University, C.F. Møllers Alle 3, 8000C, Aarhus, Denmark.

Nature
|March 1, 2013
PubMed
まとめ

円形のRNA (circRNAs) は,miRNAのスポンジとして作用することができる. 新しいcircRNA,ciRS-7は,マイクロRNA-7 (miR-7) の強力なスポンジとして機能し,脳内の遺伝子発現に影響を与えます.

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MicroRNA-based Regulation of Picornavirus Tropism
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MicroRNA-based Regulation of Picornavirus Tropism

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Single-step Purification of Macromolecular Complexes Using RNA Attached to Biotin and a Photo-cleavable Linker
08:12

Single-step Purification of Macromolecular Complexes Using RNA Attached to Biotin and a Photo-cleavable Linker

Published on: January 3, 2019

関連する実験動画

Last Updated: May 13, 2026

Highly Efficient Ligation of Small RNA Molecules for MicroRNA Quantitation by High-Throughput Sequencing
14:15

Highly Efficient Ligation of Small RNA Molecules for MicroRNA Quantitation by High-Throughput Sequencing

Published on: November 18, 2014

MicroRNA-based Regulation of Picornavirus Tropism
09:05

MicroRNA-based Regulation of Picornavirus Tropism

Published on: February 6, 2017

Single-step Purification of Macromolecular Complexes Using RNA Attached to Biotin and a Photo-cleavable Linker
08:12

Single-step Purification of Macromolecular Complexes Using RNA Attached to Biotin and a Photo-cleavable Linker

Published on: January 3, 2019

科学分野:

  • 分子生物学は分子生物学である.
  • 遺伝学 遺伝学とは
  • RNA 生物学 RNA 生物学

背景:

  • マイクロRNA (miRNA) は,遺伝子発現を転写後に制御する.
  • miRNAの活動は,競合する内生RNA (ceRNAs) とターゲットミミクリによって調節され得る.
  • 円形のRNA (circRNAs) は,規制の可能性のあるRNA分子の一種である.

研究 の 目的:

  • 人とマウスの脳における高度に発現するcircRNAの機能を調査する.
  • このcircRNAがmiRNAのスポンジとして作用するかどうかを判断する.
  • circRNA媒介のmiRNAスポンジング in vivoの影響を調査する.

主な方法:

  • 新しいcircRNA (ciRS-7) の識別と特徴付け.
  • アルゴナウト (AGO) タンパク質とmiR-7とのciRS-7の相互作用の評価.
  • miR-7の活性と標的遺伝子発現に対するciRS-7の効果の分析.
  • マウスの脳におけるciRS-7とmiR-7のインビボ共発現分析.
  • miRNAのスポンジとして別のcircRNA (Sry) の調査.

主要な成果:

  • 新しいcircRNA,ciRS-7が特定され,多数のmiR-7標的部位を含むことが示されました.
  • ciRS-7は,miR-7に依存した方法で,AGOタンパク質と結合する.
  • ciRS-7は強力なmiR-7スポンジとして機能し,miR-7の活性を抑制し,miR-7の標的レベルを上昇させます.
  • ciRS-7とmiR-7の重複発現は,マウスの特定の脳の領域で観察されました.
  • circRNA SryはmiR-138スポンジとして作用し,一般的な現象を示していることが実証されました.

結論:

  • 円形のRNAは,内生的なmiRNAスポンジとして機能することができます.
  • ciRS-7は,miR-7の機能的なスポンジであり,脳内の活動を調節する.
  • CircRNA形成は,miRNAのスポンジ化と遺伝子調節のための一般的なメカニズムを表しています.