関連する実験動画
Updated: May 13, 2026
08:25
Identification of Circular RNAs using RNA Sequencing
Published on: November 14, 2019
循環型RNAは,動物性RNAの大きなクラスで,規制的な効能を持つRNAです
Sebastian Memczak1, Marvin Jens, Antigoni Elefsinioti
1Systems Biology of Gene Regulatory Elements, Max-Delbrück-Center for Molecular Medicine, Robert-Rössle-Strasse 10, 13125 Berlin, Germany.
Nature
|March 1, 2013
まとめ
円形のRNA (circRNAs) は,新たに発見されたRNA分子の一種である. これらの安定した分子は,遺伝子発現に影響を与えるマイクロRNA (miRNA) を結合することによって,重要な転写後の調節体として作用します.
科学分野:
- 分子生物学は分子生物学である.
- 遺伝学 遺伝学とは
- 発達生物学 発達生物学とは
背景:
- 円形のRNA (circRNAs) は,ほとんど未知の機能を持つ謎のRNA分子クラスを表しています.
- その安定性と潜在的な規制的役割は,体系的な調査を必要とします.
研究 の 目的:
- 異なる種で循環型RNA (circRNAs) を体系的に特定し,分析する.
- サークRNAの機能的役割と規制の可能性,特に転写後の調節に関する研究を行う.
主な方法:
- 人体,マウス,ネマトード標本の高通量RNA配列解析.
- サークRNAを検出し,特徴づけるための計算分析.
- セブラフィッシュにおける機能的測定は,circRNAsが発達に与える影響を評価するために行われます.
主要な成果:
- 何千もの安定した,よく発現するcircRNAが特定され,その多くは組織および発達段階に特異的な発現パターンを表している.
- ヒトの特定のcircRNAであるCDR1asは,多数のマイクロRNA (miRNA) エフェクター複合体を結合し,特にmiR-7の強力なスポンジとして作用することが判明しました.
- CDR1asはmiRNAアンタゴニストとして機能し,ゼブラフィッシュにおける実験的な証拠は,中脳発達調節におけるその役割を実証しています.
結論:
- 円形RNA (circRNAs) は,転写後のレギュレータの重要なクラスである.
- サークRNA形成におけるエクソンのヘッド・トゥ・テール・スプライシングは,コーディングシーケンスから派生した新しい規制の可能性を示唆する.
関連する概念動画
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Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
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01:23Types of RNA
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Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
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01:20Types of RNA
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
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01:20Types of RNA
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
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01:23Types of RNA
Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
01:23RNA Interference
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...