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関連する概念動画

Transcription Factors02:16

Transcription Factors

70.9K
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
70.9K
Histone Modification02:32

Histone Modification

14.7K
The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone...
14.7K
Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

6.6K
In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
6.6K
Co-activators and Co-repressors02:04

Co-activators and Co-repressors

7.0K
Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
7.0K
Master Transcription Regulators02:23

Master Transcription Regulators

6.1K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
6.1K
General Transcription Factors01:30

General Transcription Factors

5.9K
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
5.9K

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関連する実験動画

Updated: May 6, 2026

Isolation and Cultivation of Neural Progenitors Followed by Chromatin-Immunoprecipitation of Histone 3 Lysine 79 Dimethylation Mark
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Isolation and Cultivation of Neural Progenitors Followed by Chromatin-Immunoprecipitation of Histone 3 Lysine 79 Dimethylation Mark

Published on: January 26, 2018

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TAF3とのH3K4me3の相互作用は,前始動複合体の構成と選択的な遺伝子活性化を調節する.

Shannon M Lauberth1, Takahiro Nakayama, Xiaolin Wu

  • 1Laboratory of Biochemistry and Molecular Biology, The Rockefeller University, New York, NY 10065, USA.

Cell
|March 5, 2013
PubMed
まとめ

ヒストンマークH3K4me3は,転写因子TFIIDを活性遺伝子に誘導し,p53-駆動遺伝子発現を強化する. このメカニズムは,遺伝子毒性ストレス中に迅速なp53標的遺伝子の誘導を保証します.

さらに関連する動画

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers
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Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers

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HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries
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HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries

Published on: March 31, 2019

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関連する実験動画

Last Updated: May 6, 2026

Isolation and Cultivation of Neural Progenitors Followed by Chromatin-Immunoprecipitation of Histone 3 Lysine 79 Dimethylation Mark
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Isolation and Cultivation of Neural Progenitors Followed by Chromatin-Immunoprecipitation of Histone 3 Lysine 79 Dimethylation Mark

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Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers
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Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers

Published on: September 20, 2018

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HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries
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科学分野:

  • 分子生物学は分子生物学である.
  • エピジェネティクス エピジェネティクス
  • 遺伝子規制 遺伝子規制

背景:

  • ヒストンの改変が染色体ベースのプロセスを制御しますが,それらの特定の役割は完全に理解されていません.
  • リジン4 (H3K4me3) でトリメチル化ヒストロンH3は,活性遺伝子と関連しており,TFIIDのようなエフェクタタンパク質経由で転写を助けます.

研究 の 目的:

  • H3K4me3が遺伝子転写に影響を与えるメカニズム,特にp53標的遺伝子の文脈で解明する.
  • TFIIDの募集と事前開始複合体の形成を指揮するH3K4me3-TAF3の相互作用の役割を調査する.

主な方法:

  • TFIIDの成分であるH3K4me3とTAF3の相互作用を調査した.
  • H3K4me3がp53-依存転写と前始動複合体 (PIC) 形成に与える影響を分析した.
  • 遺伝子調節におけるH3K4me3,TAF3,TATAボックス,PICアセンブリの相互作用を調べました.

主要な成果:

  • H3K4me3-TAF3の相互作用は,p53ターゲットを含む活性遺伝子に対する世界的なTFIIDの徴募に不可欠です.
  • H3K4me3は,PIC形成を促進することによって,p53-依存トランスクリプションを強化し,独立してまたはTATAボックスで作用します.
  • H3K4me3-TAF3/TFIIDの相互作用は,遺伝子毒性ストレスへの反応としてp53の遺伝子選択機能を調節する.

結論:

  • H3K4me3は,TAF3/TFIIDの相互作用を通じてPICアセンブリを指揮するキーレギュレータとして機能します.
  • このメカニズムは,遺伝子毒性ストレスによる特定のp53標的遺伝子の急速な誘導を促進します.
  • この研究は,遺伝子転写と細胞応答の表遺伝子制御のための新しい経路を明らかにしています.