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関連する概念動画

Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or quantified.
Photoluminescence: Applications01:14

Photoluminescence: Applications

Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...

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関連する実験動画

Updated: May 13, 2026

Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds
10:10

Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds

Published on: November 13, 2021

均質な化学発光免疫測定法である.

Hashem Akhavan-Tafti1, Dean G Binger, John J Blackwood

  • 1Beckman Coulter Inc., Southfield, Michigan 48033, USA. hakhavantafti@beckman.com

Journal of the American Chemical Society
|March 13, 2013
PubMed
まとめ

この研究では,新しい均質な化学発光免疫測定法が導入されました. この方法は,ラベル付きの結合部位とトリガー溶液を使用して,分離することなく直接分析量を定量化します.

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A Sensitive and Specific Quantitation Method for Determination of Serum Cardiac Myosin Binding Protein-C by Electrochemiluminescence Immunoassay
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A Sensitive and Specific Quantitation Method for Determination of Serum Cardiac Myosin Binding Protein-C by Electrochemiluminescence Immunoassay

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Procedure and Key Optimization Strategies for an Automated Capillary Electrophoretic-based Immunoassay Method
09:32

Procedure and Key Optimization Strategies for an Automated Capillary Electrophoretic-based Immunoassay Method

Published on: September 10, 2017

関連する実験動画

Last Updated: May 13, 2026

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10:10

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Published on: November 13, 2021

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科学分野:

  • バイオケミストリー バイオケミストリー
  • アナリティカル・ケミストリー (Analytical Chemistry) とは
  • 免疫化学 免疫化学とは

背景:

  • 化学発光免疫測定法 (CIA) は,分析物質の検出に広く使用されています.
  • 均質なCIAは,分離のステップを排除することで利点を提供します.
  • 敏感で効率的な均質なCIAの方法の開発は,診断に不可欠です.

研究 の 目的:

  • 新しい均質な化学発光免疫測定法 (CIA) 方法について報告する.
  • 化学発光化合物とペロキシダゼでラベルを貼った特定の結合メンバーを活用するために.
  • 分離を必要とせずに,直接の分析量化を達成するために.

主な方法:

  • 均質な化学発光免疫測定法が開発されました.
  • 特定の結合部位には,アクリダン基の化学発光化合物とペロキシダースのラベルが付けられました.
  • 免疫複合体の形成により,ラベルが近接し,トリガー溶液の添加時に信号を生成します.

主要な成果:

  • 測定は均一に機能し,分離ステップの必要性を排除します.
  • トリガー溶液を加えると,化学発光信号が生成されます.
  • 信号の強さは,分析物質の濃度と直接相関しています.

結論:

  • 報告された方法は,敏感で効率的な均質な化学発光免疫測定法を提供します.
  • この測定形式は,分離ステップを削除することによって,分析物質の検出を簡素化します.
  • 信号と分析量間の直接的な相関は,正確な定量化が可能である.