ディペプチジルペプチダゼ4は,新興の人間の新型コロナウイルス-EMCの機能的受容体です
V Stalin Raj1, Huihui Mou, Saskia L Smits
1Department of Viroscience, Erasmus Medical Center, 3000 CA Rotterdam, The Netherlands.
Nature
|March 15, 2013
まとめ
研究者は,ヒトコロナウイルス-エラスマス医療センター (hCoV-EMC) の細胞受容体としてディペプチジルペプチダゼ4 (DPP4) を特定しました. この発見は,ウイルスの洞察力を提供します.
科学分野:
- ウイルス学 ウイルス学 ウイルス学
- 感染症 感染症は感染症です.
- 分子生物学は分子生物学である.
背景:
- 人間のコロナウイルスは通常,軽度の呼吸器疾患を引き起こす.
- SARSやhCoV-EMCのような新興コロナウイルスは,重症で致死的な下呼吸道感染を引き起こす可能性があります.
- hCoV-EMCは,コウモリコロナウイルスと遺伝的類似性を共有しています.
研究 の 目的:
- hCoV-EMC.の細胞受容体を特定する.
- hCoV-EMC.の宿主範囲と病原性を理解する.
- 介入戦略の開発に情報を提供するためです.
主な方法:
- DPP4とhCoV-EMCのスパイクタンパク質S1ドメインの共同浄化.
- 抗-DPP4抗体を用いてhCoV-EMC感染を抑制する.
- 感染性をテストするために,ヒトおよびコウモリDPP4の非感受性細胞の発現.
主要な成果:
- ディペプチジルペプチダゼ4 (DPP4) は,hCoV-EMC.の機能的受容体として特定されました.
- DPP4は,hCoV-EMCスパイクタンパク質のS1ドメインに特異的に結合する.
- アンチ-DPP4抗体は,細胞培養におけるhCoV-EMC感染を阻害した.
- 人間とコウモリのDPP4は,非許容性細胞のhCoV-EMC感染を可能にしました.
結論:
- DPP4は,hCoV-EMCの細胞侵入と宿主トロピズムを決定する重要な決定因子です.
- 種間のDPP4の保存された性質は,hCoV-EMCの広範囲の宿主範囲の可能性を示唆しています.
- DPP4-hCoV-EMCの相互作用を理解することは,抗ウイルス療法を開発し,病気の拡散を予測するために重要です.
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