関連する実験動画

Updated: May 13, 2026

Production, Crystallization, and Structure Determination of the IKK-binding Domain of NEMO
13:02

Production, Crystallization, and Structure Determination of the IKK-binding Domain of NEMO

Published on: December 28, 2019

NF-κB基本調節器/IKKβタンパク質-タンパク質インターフェースの結合エネルギーホットスポットの包括的な実験および計算分析

Mary S Golden1, Shaun M Cote, Marianna Sayeg

  • 1Department of Chemistry, Boston University, 590 Commonwealth Avenue, Boston, Massachusetts 02215, USA.

Journal of the American Chemical Society
|March 20, 2013
PubMed
まとめ

研究者は,核因子カッパB (NF-κB) エッセンシャルモジュレーター (NEMO) とIκBキナーゼサブユニットβ (IKKβ) インターフェースの結合エネルギーホットスポットを分析した. この研究は,重要な残留物を特定し,この重要なシグナル伝達経路を標的とした薬剤発見のための計算方法を検証します.

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A Guide to Production, Crystallization, and Structure Determination of Human IKK1/α

Published on: November 2, 2018

NF-κB-dependent Luciferase Activation and Quantification of Gene Expression in Salmonella Infected Tissue Culture Cells10:57

NF-κB-dependent Luciferase Activation and Quantification of Gene Expression in Salmonella Infected Tissue Culture Cells

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The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
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Conserved Binding Sites01:49

Conserved Binding Sites

Many proteins’ biological role depends on their interactions with their ligands, small molecules that bind to specific locations on the protein known as ligand-binding sites. Ligand-binding sites are often conserved among homologous proteins as these sites are critical for protein function.
Binding sites are often located in large pockets, and if their location on a protein’s surface is unknown, it can be predicted using various approaches. The energetic method computationally analyses the...
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