CRISPR/Casシステムは,バクテリアの先天性免疫回避と毒性を媒介する

Timothy R Sampson¹, Sunil D Saroj, Anna C Llewellyn

¹Microbiology and Molecular Genetics Program, Department of Microbiology and Immunology, Emory University, Atlanta, Georgia 30329, USA.

Nature

|April 16, 2013

まとめ

フランシセラ・ノビシダのCRISPR/Casシステムは,細菌のリポタンパク質遺伝子を抑制するために小さなRNAを使用し,宿主の免疫反応を回避し,病原体の毒性を促進するために不可欠です.

科学分野:

微生物学微生物学とは
バクテリアの遺伝学
分子生物学は分子生物学である.

背景:

CRISPR/Casシステムは,外来DNAに対する細菌の免疫メカニズムです.
内生細菌の遺伝子を調節する彼らの役割は,ほとんど未知のままです.
細菌のリポタンパク質は宿主の炎症反応を誘発する可能性があります.

研究の目的:

防御不能のFrancisella novicidaにおけるCRISPR/Casシステムの機能を調査する.
CRISPR/Casが内生的な遺伝子発現を調節するかどうかを判断する.
バクテリアの毒性におけるCRISPR/Cas媒介遺伝子調節の役割を明らかにする.

主な方法:

新型の小型のCRISPR/Cas関連RNA (scaRNA) の識別と特徴付け.
内生性トランスクリプトへのスカRNA結合の実証.
バクテリアのリポタンパク質発現に対するscaRNAの調節効果の分析.

主要な成果:

フランシセラ・ノビシダは,Cas9タンパク質とユニークなスカRNAを使用しています.
このシステムは,細菌のリポタンパク質をコードする内生性トランスクリプトを抑制します.
この抑制は,F. novicidaが宿主の炎症反応を抑制し,毒性を高めるために不可欠です.

結論:

CRISPR/Casシステムは,内生細菌の遺伝子発現を調節することができます.
バクテリアのリポタンパク質のCas9媒介抑制は,F. novicidaの毒性にとって非常に重要です.
CRISPR/Cas媒介遺伝子調節は,細菌に広く存在し,宿主相互作用に影響を与える可能性があります.

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