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Updated: May 12, 2026

Anaerobic Protein Purification and Kinetic Analysis via Oxygen Electrode for Studying DesB Dioxygenase Activity and Inhibition
08:31

Anaerobic Protein Purification and Kinetic Analysis via Oxygen Electrode for Studying DesB Dioxygenase Activity and Inhibition

Published on: October 3, 2018

活性部位飽和変異による二機能的非ヘム鉄酸化酵素酸カルバペネム合成酵素に対する機械的洞察

Ryan M Phelan1, Craig A Townsend

  • 1Department of Chemistry, Johns Hopkins University, 3400 North Charles Street, Baltimore, Maryland 21218, USA.

Journal of the American Chemical Society
|April 25, 2013
PubMed
まとめ

カーバペネム合成酵素 (CarC) は,C5のエピメリゼーションと脱飽和を経て単純なカーバペネムを生成する. この酵素は2つの基板ステレオアイソマーを1つの抗生物質に処理し,2段階の反応機構を示唆しています.

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A Protocol for Functional Assessment of Whole-Protein Saturation Mutagenesis Libraries Utilizing High-Throughput Sequencing11:36

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