関連する実験動画
Updated: May 11, 2026

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Detection of Alternative Splicing During Epithelial-Mesenchymal Transition
Published on: October 9, 2014
哺乳類の細胞におけるタンパク質・スキャフォールド活性化タンパク質のトランススプライシング
Daniel F Selgrade1, Jason J Lohmueller, Florian Lienert
1Department of Systems Biology, Harvard Medical School, Boston, Massachusetts 02115, USA.
Journal of the American Chemical Society
|April 30, 2013
まとめ
研究者らは,哺乳類の細胞内の酵素活性を制御するための新しいタンパク質・スカファード・システムを開発した. このタンパク質誘発スプライシング方法は,のルシフェラーゼを活性化させ,バイオセンサと規制システムに対する強化された遺伝子制御を提供します.
科学分野:
- バイオテクノロジー バイオテクノロジー
- 分子生物学は分子生物学である.
- 合成生物学 合成生物学とは
背景:
- 条件付きタンパク質スプライシングは,タンパク質の活性に対する正確な翻訳後の制御を提供します.
- 既存の方法は,特定の化学的または環境的トリガーを必要とする.
研究 の 目的:
- 遺伝子でコードされたタンパク質の支架によって制御される新しい条件付きタンパク質スプライシングシステムを開発する.
- 哺乳類の細胞における酵素のスキャフォールド誘発活性化を実証する.
主な方法:
- アンチパラレル・コイルドコイル (CC) を使用したエンジニアリングされた分割インテイン.
- 2つのスカフォールド誘発スプライシングシステムを構築し,テストしました.
- リンカーの長さを最適化し,酵素活性化を測定しました.
主要な成果:
- CCs.によって仲介された機能的な分割インテインを成功裏に作成しました.
- スキャフォルド誘発のスプライシングと火のルシフェラーゼの活性化が実証されています.
- コントロールと比較して,ルシフェラーゼの活性が13倍増加した.
結論:
- タンパク質・スキャフォルド・トリガード・システムは,タンパク質の入力による酵素活性制御のための新しい方法を提供します.
- このシステムは,タンパク質のスプライシングに対する遺伝的制御を強化します.
- 潜在的な応用には,スプライシングベースのタンパク質センサーと自己調節システムが含まれます.
関連する概念動画
Assembly of Signaling Complexes
Multiprotein signaling complexes are formed in a dynamic process involving protein-protein interactions at the cytoplasmic domain of transmembrane receptors or enzymatic and non-enzymatic proteins associated with the receptor. These complexes ensure the activation and propagation of intracellular signals that regulate cell functions.
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...
Alternative RNA Splicing
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
RNA Splicing
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
MAPK Signaling Cascades
Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
TGF - β Signaling Pathway
The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors are of three kinds RI, RII, and RIII. The RI...

